Curcumin administration suppress acetylcholinesterase gene expression in cadmium treated rats

• Published in: Neurotoxicology (Park Forest South) 2017-09, Vol.62, p.75-79
• Main Authors: Akinyemi, Ayodele Jacob, Oboh, Ganiyu, Fadaka, Adewale Oluwaseun, Olatunji, Babawale Peter, Akomolafe, Seun
• Format: Article
• Language: English
• Subjects: Acetylcholinesterase; Acetylcholinesterase - genetics; Acetylcholinesterase - metabolism; Alzheimer's disease; Animals; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Brain; Cadmium; Cadmium - pharmacology; Cerebral cortex; Cerebral Cortex - drug effects; Cerebral Cortex - enzymology; Curcumin; Curcumin - pharmacology; Dose-Response Relationship, Drug; Gene expression; Gene Expression Regulation - drug effects; Male; mRNA gene expression; Neurodegenerative diseases; Neurological diseases; Polymerase chain reaction; Rats; Rhizomes; RNA, Messenger - metabolism; RNA-directed DNA polymerase; Rodents
• ISSN: 0161-813X; 1872-9711
• DOI: 10.1016/j.neuro.2017.05.004

•Curcumin prevents Cd neurotoxicity.•Curcumin exhibited inhibitory effect on acetycholinesterase activity.•Curcumin suppress acetylcholinesterase gene expression in cadmium treated rats. Curcumin, the main polyphenolic component of turmeric (Curcuma longa) rhizomes have been reported to exert anticholinesterase potential with limited information on how they regulate acetylcholinesterase (AChE) gene expression. Hence, this study sought to evaluate the effect of curcumin on cerebral cortex acetylcholinesterase (AChE) activity and their mRNA gene expression level in cadmium (Cd)-treated rats. Furthermore, in vitro effect of different concentrations of curcumin (1–5μg/mL) on rat cerebral cortex AChE activity was assessed. Animals were divided into six groups (n=6): group 1 serve as control (without Cd) and receive saline/vehicle, group 2 receive saline plus curcumin at 25mg/kg, group 3 receive saline plus curcumin 50mg/kg, group 4 receive Cd plus vehicle, group 5 receive Cd plus curcumin at 25mg/kg and group 6 receive Cd plus curcumin at 50mg/kg. Rats received Cd (2.5mg/kg) and curcumin (25 and 50mg/kg, respectively) by oral gavage for 7days. Acetylcholinesterase activity was measured by Ellman’s method and AChE expression was carried out by a quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) assay. We observed that acute administration of Cd increased acetylcholinesterase activity and in addition caused a significant (P