Wound healing and anti-inflammatory activity of some Ononis taxons

• Published in: Biomedicine & pharmacotherapy 2017-07, Vol.91, p.1096-1105
• Main Authors: Ergene Öz, Burçin, Saltan İşcan, Gülçin, Küpeli Akkol, Esra, Süntar, İpek, Keleş, Hikmet, Bahadır Acıkara, Özlem
• Format: Article
• Language: English
• Subjects: Analgesics - pharmacology; Animals; Anti-Inflammatory Agents - pharmacology; Antioxidants - pharmacology; Benzothiazoles - pharmacology; Biphenyl Compounds - pharmacology; Edema - chemically induced; Edema - drug therapy; Fabaceae; Hydroxides - chemistry; Inflammation; Inflammation - chemically induced; Inflammation - drug therapy; Internal Medicine; Male; Medical Education; Methanol - chemistry; Mice; Ononis; Ononis - chemistry; Phytotherapy - methods; Picrates - pharmacology; Plant Extracts - pharmacology; Plant Roots - chemistry; Rats; Rats, Sprague-Dawley; Skin - drug effects; Sulfonic Acids - pharmacology; Wound healing; Wound Healing - drug effects
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2017.05.040

Abstract Ononis species are used for their laxative, diuretic, analgesic, anti-inflammatory, antiviral, cytotoxic and antifungal effects as well as against skin diseases for wound healing activity. In the light of this information n- hexane, ethylacetate and methanol extracts prepared from Ononis spinosa L. subsp. leiosperma (Boiss.) Sirj. , Ononis variegata L. , Ononis viscosa L. subsp. brevifolia (DC) Nym. and Ononis natrix L. subsp. natrix L. were tested for their wound healing, anti-inflammatory and antioxidant activities. Linear incision and circular excision wound models and hydroxypyroline estimation assay were used for the wound healing activity. For the assessment of chronic inflammation FCA-induced arthritis and for acute inflammation carrageenan-induced hind paw edema, TPA-induced ear edema and acetic acid-induced increase in capillary permeability tests were conducted. 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) scavenging activity assay, reducing power assay and hydroxyl radical (OH− ) scavenging assay were used for determining antioxidant activities of the extracts. Results showed that O. spinosa subsp. leiosperma roots ethyl acetate extract exhibited remarkable wound healing activity with the 42.6% tensile strength value on the linear incision wound model and 60.1% reduction of the wound area at the day 12 on the circular excision wound model. Hydroxyproline content of the tissue treated by O. spinosa subsp. leiosperma roots ethyl acetate extract was found to be 41.3 μg/mg. Acetic acid induced increase in capillary permeability test results revealed that O. spinosa subsp. leiosperma roots ethyl acetate extract and O. spinosa subsp. leiosperma roots methanol extract inhibited inflammation by 40.4% and 35.4% values respectively. O. spinosa subsp. leiosperma roots ethyl acetate extract showed 21.2-27.2% inhibition in carrageenan-induced hind paw edema test while did not posses activity on TPA-induced ear edema and FCA-induced arthritis models.