Identification of Methylation Profiles of Cancer-related Genes in Circulating Tumor Cells Population

We performed an epigenetic analysis of the first exon of the hVIM gene and the SFRP2 in circulating tumor cells (CTCs) and correlation with the corresponding primary colorectal cancer (CRC) tissue. CTCs detection in 52 colorectal cancer patients was managed by a multi-marker immunomagnetic method wi...

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Bibliographic Details
Published in:Anticancer research 2017-03, Vol.37 (3), p.1105-1112
Main Authors: Lyberopoulou, Anna, Galanopoulos, Michail, Aravantinos, Gerasimos, Theodoropoulos, George E, Marinos, Evangelos, Efstathopoulos, Efstathios P, Gazouli, Maria
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Case-Control Studies
Chromosome Aberrations
Colorectal Neoplasms - blood
Colorectal Neoplasms - genetics
DNA Methylation
Epigenesis, Genetic
Female
Genotype
Humans
Male
Middle Aged
Mutation
Neoplastic Cells, Circulating
Oligonucleotide Array Sequence Analysis
Oncogenes
Promoter Regions, Genetic
Proto-Oncogene Proteins B-raf - genetics
Proto-Oncogene Proteins p21(ras) - genetics
Quantum Dots
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Summary:We performed an epigenetic analysis of the first exon of the hVIM gene and the SFRP2 in circulating tumor cells (CTCs) and correlation with the corresponding primary colorectal cancer (CRC) tissue. CTCs detection in 52 colorectal cancer patients was managed by a multi-marker immunomagnetic method with the use of quantum dots (QDs). To determine methylation levels we used high-resolution melting (HRM) technology. In the case of VIM we found 76.9% methylated samples, compared to 53.8% in tissue samples. Regarding SFRP2 promoter methylation levels in tissue and CTCs samples, 67.3% and 73.1%, were found methylated respectively. Correlation analysis of methylation levels with KRAS and BRAF mutations (performed in our previous study) demonstrates that high-methylation epigenotype strongly correlates to BRAF mutation. CTCs is a promising diagnostic tool. The combination of genetic mutations and epigenetic aberrations specifically in CTCs, will ameliorate CRC diagnosis in the future.
ISSN:0250-7005
1791-7530
DOI:10.21873/anticanres.11423