Metabolic Effect of 1‑Deoxynojirimycin from Mulberry Leaves on db/db Diabetic Mice Using Liquid Chromatography–Mass Spectrometry Based Metabolomics

Metabolomics was applied to the liquid chromatography–mass spectrometry urinary metabolic profile of type 2 diabetes (T2DM) mice treated with mulberry 1-deoxynojirimycin (DNJ). The serum biochemical indicators related to T2DM like blood glucose, insulin, triglyceride, total cholesterol, nitrogen, ma...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2017-06, Vol.65 (23), p.4658-4667
Main Authors: Hu, Xue-Qin, Thakur, Kiran, Chen, Gui-Hai, Hu, Fei, Zhang, Jian-Guo, Zhang, Hong-Bin, Wei, Zhao-Jun
Format: Article
Language:English
Subjects:
1-Deoxynojirimycin - administration & dosage
1-Deoxynojirimycin - analysis
1-Deoxynojirimycin - metabolism
Animals
Blood Glucose
Chromatography, High Pressure Liquid
Diabetes Mellitus, Type 2 - drug therapy
Diabetes Mellitus, Type 2 - metabolism
Humans
Hypoglycemic Agents - administration & dosage
Hypoglycemic Agents - analysis
Hypoglycemic Agents - metabolism
Insulin
Male
Mass Spectrometry
Metabolomics
Mice
Mice, Inbred C57BL
Morus - chemistry
Morus - metabolism
Plant Extracts - administration & dosage
Plant Extracts - chemistry
Plant Extracts - metabolism
Plant Leaves - chemistry
Plant Leaves - metabolism
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Summary:Metabolomics was applied to the liquid chromatography–mass spectrometry urinary metabolic profile of type 2 diabetes (T2DM) mice treated with mulberry 1-deoxynojirimycin (DNJ). The serum biochemical indicators related to T2DM like blood glucose, insulin, triglyceride, total cholesterol, nitrogen, malondialdehyde, and creatinine decreased significantly in the treated group. The histopathological changes in liver cells were marked by deformations and disruptions in central area of nuclei in DM mice, whereas DNJ treatment recovered regular liver cells with normal nuclei. Most of the metabolites of T2DM were significantly different from healthy controls in the bulk data generated. The level of 16 metabolites showed that the diabetic group was closer to the healthy group as the DNJ treatment time prolonged. Moreover, DNJ inhibited the activity of glucosidase on glucose, lipid, and amino acid metabolism. Our results showed the mechanism of DNJ treatment of T2DM and could fetch deep insights into the potent metabolite markers of the applied antidiabetic interventions.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.7b01766