Cell cycle synchronization and analysis of apoptosis‐related gene in skin fibroblasts from domestic cat (Felis silvestris catus) and kodkod (Leopardus guigna)

Contents The kodkod population is in constant decrease and the somatic cell nuclear transfer (SCNT) might help to preserve the genetic pool of this species. The cell cycle synchronization of donor cells plays a crucial role in SCNT. The objective of this research was to evaluate two different method...

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Bibliographic Details
Published in:Reproduction in domestic animals 2017-10, Vol.52 (5), p.881-889
Main Authors: Veraguas, D, Gallegos, PF, Castro, FO, Rodriguez‐Alvarez, L
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Apoptosis - genetics
Cats
Cell cycle
Cell Cycle - physiology
Cell Survival
Cloning, Organism - veterinary
Contact Inhibition
Culture Media, Serum-Free
Cytometry
Felidae - physiology
Fibroblasts
Fibroblasts - cytology
Flow cytometry
flow cytometry analysis
Gene expression
Gene Expression Profiling
Nuclear transfer
Nuclear Transfer Techniques - veterinary
Resting Phase, Cell Cycle
Skin
Somatic cell nuclear transfer
Synchronism
Synchronization
wild felids
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Summary:Contents The kodkod population is in constant decrease and the somatic cell nuclear transfer (SCNT) might help to preserve the genetic pool of this species. The cell cycle synchronization of donor cells plays a crucial role in SCNT. The objective of this research was to evaluate two different methods for quiescence induction, serum starvation (SS) and contact inhibition (CI), both for 1, 3 and 5 days, on skin fibroblast from domestic cat and kodkod. Flow cytometry analysis revealed that in domestic cat, SS and CI, both at 3 and 5 days, increased the percentage of fibroblasts in G0/G1 compared to growing cells (GC) (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.12994