Detection of Nitric Oxide from Living Cells Using Polymeric Zinc Organic Framework‐Derived Zinc Oxide Composite with Conducting Polymer

• Published in: Small (Weinheim an der Bergstrasse, Germany) Germany), 2017-07, Vol.13 (26), p.n/a
• Main Authors: Kim, Min‐Yeong, Naveen, Malenahalli Halappa, Gurudatt, Nanjanagudu Ganesh, Shim, Yoon‐Bo
• Format: Article
• Language: English
• Subjects: Accessibility; Benzoic acid; Biotechnology; Cells (biology); Conducting polymers; dithiooxamide; Dynamic range; Electrical measurement; Electrolytic cells; Electron transport; Feasibility studies; Graphene; Human body; living cells; Nanocomposites; Nanotechnology; Nitric oxide; nitric oxide sensors; Real time; Sensors; Stimulation; Surface area; Zinc oxide; Zinc oxides
• ISSN: 1613-6810; 1613-6829
• DOI: 10.1002/smll.201700502

Sensitive and selective detection of nitric oxide (NO) in the human body is crucial since it has the vital roles in the physiological and pathological processes. This study reports a new type of electrochemical NO biosensor based on zinc‐dithiooxamide framework derived porous ZnO nanoparticles and polyterthiophene‐rGO composite. By taking advantage of the synergetic effect between ZnO and poly(TTBA‐rGO) (TTBA = 3′‐(p‐benzoic acid)‐2,2′:5′,2″‐terthiophene, rGO = reduced graphene oxide) nanocomposite layer, the poly(TTBA‐rGO)/ZnO sensor probe displays excellent electrocatalytic activity and explores to detect NO released from normal and cancer cell lines. The ZnO is immobilized on a composite layer of poly(TTBA‐rGO). The highly porous ZnO offers a high electrolyte accessible surface area and high ion–electron transport rates that efficiently catalyze the NO reduction reaction. Amperometry with the modified electrode displays highly sensitive response and wide dynamic range of 0.019–76 × 10−6m with the detection limit of 7.7 ± 0.43 × 10−9m. The sensor probe is demonstrated to detect NO released from living cells by drug stimulation. The proposed sensor provides a powerful platform for the low detection limit that is feasible for real‐time analysis of NO in a biological system. A new type of electrochemical nitric oxide (NO) biosensor based on zinc‐dithiooxamide framework derived porous zinc oxide (ZnO) nanoparticles and polyterthiophene‐reduced graphene oxide (rGO) composite is developed. The porous zinc oxide efficiently catalyzes the NO reduction reaction. The polyterthiophene‐rGO layer gives a stable electrode surface. The sensor probe displays excellent electrocatalytic activity for the sensitive detection of NO from living cells.