A shortened, two-enzyme pathway for 2,3-butanediol production in Escherichia coli

The platform chemical 2,3-butanediol (2,3-BDO) is produced by a number of microorganisms via a three-enzyme pathway starting from pyruvate. Here, we report production of 2,3-BDO via a shortened, two-enzyme pathway in Escherichia coli . A synthetic operon consisting of the acetolactate synthase (ALS)...

Full description

Saved in:
Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2017-09, Vol.44 (9), p.1273-1277
Main Authors: Reshamwala, Shamlan M. S., Deb, Shalini S., Lali, Arvind M.
Format: Article
Language:English
Subjects:
Acetoin
Acetoin - metabolism
Acetolactate synthase
Acetolactate Synthase - genetics
Acetolactate Synthase - metabolism
Alcohol Oxidoreductases - genetics
Alcohol Oxidoreductases - metabolism
Biochemistry
Bioenergy/Biofuels/Biochemicals - Short Communication
Bioinformatics
Biomedical and Life Sciences
Bioreactors
Biosynthesis
Biotechnology
Butanediol
Butylene Glycols - metabolism
Cultivation
E coli
Enterobacter - enzymology
Enterobacter - genetics
Enzymes
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli - metabolism
Genes
Genetic Engineering
Genomes
Inorganic Chemistry
Life Sciences
Microbiology
Microorganisms
Operon - genetics
Plasmids - genetics
Pyruvic acid
Reductase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The platform chemical 2,3-butanediol (2,3-BDO) is produced by a number of microorganisms via a three-enzyme pathway starting from pyruvate. Here, we report production of 2,3-BDO via a shortened, two-enzyme pathway in Escherichia coli . A synthetic operon consisting of the acetolactate synthase (ALS) and acetoin reductase (AR) genes from Enterobacter under control of the T7 promoter was cloned in an episomal plasmid. E. coli transformed with this plasmid produced 2,3-BDO and the pathway intermediate acetoin, demonstrating that the shortened pathway was functional. To assemble a synthetic operon for inducer- and plasmid-free production of 2,3-BDO, ALS and AR genes were integrated in the E. coli genome under control of the constitutive ackA promoter. Shake flask-level cultivation led to accumulation of ~1 g/L acetoin and ~0.66 g/L 2,3-BDO in the medium. The novel biosynthetic route for 2,3-BDO biosynthesis described herein provides a simple and cost-effective approach for production of this important chemical.
ISSN:1367-5435
1476-5535
DOI:10.1007/s10295-017-1957-5