Synbiotic loaded chitosan-Ca-alginate microparticles reduces inflammation in the TNBS model of rat colitis

[Display omitted] New therapeutic strategies against inflammatory bowel disease (IBD) consider the usage of probiotics, prebiotics and synbiotics as beneficial for the intestinal microbial balance. Limitations of such an approach are addressed into difference in survival, persistence, colonization a...

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Bibliographic Details
Published in:International journal of pharmaceutics 2017-07, Vol.527 (1-2), p.126-134
Main Authors: Ivanovska, Tanja Petreska, Mladenovska, Kristina, Zhivikj, Zoran, Pavlova, Maja Jurhar, Gjurovski, Ivica, Ristoski, Trpe, Petrushevska-Tozi, Lidija
Format: Article
Language:English
Subjects:
Alginates - chemistry
Animals
Chitosan - chemistry
Chitosan-Ca-alginate microparticles
Colitis - chemically induced
Colitis - drug therapy
Female
Glucuronic Acid - chemistry
Hexuronic Acids - chemistry
Inflammation - drug therapy
Lactobacillus casei 01
Oligofructose-enriched inulin
Probiotics
Rat colitis
Rats
Rats, Wistar
Synbiotics
Trinitrobenzenesulphonic acid
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Summary:[Display omitted] New therapeutic strategies against inflammatory bowel disease (IBD) consider the usage of probiotics, prebiotics and synbiotics as beneficial for the intestinal microbial balance. Limitations of such an approach are addressed into difference in survival, persistence, colonization and variable effects among different probiotic strains, lack in understanding of probiotic mechanisms of action, as well the complex etiology of IBD. The anti-inflammatory activity of Lactobacillus casei 01 (L. casei 01) was assessed in trinitrobenzenesulphonic (TNBS) acid model of rat colitis when the probiotic was used alone and/or in combination with oligofructose-enriched inulin (Synergy 1), and as synbiotic (L. casei 01+Synergy 1) loaded chitosan-Ca-alginate microparticles; all suspended in ayran. The results from the probiotic/synbiotic treatments (8.5–8.9log CFU g−1L. casei 01 and 1.5% Synergy 1) have shown reduction in the colonic damage and increased lactobacilli counts in feces. Lactobacilli translocation to sterile extra-intestinal organs demonstrated acceptable safety of the probiotic strain used. The best effect at reducing inflammation and lesions associated with a significant decline in myeloperoxidase (MPO) activity was observed in rats that received synbiotic microparticles. This finding suggests colon targeted delivery of the probiotics/synbiotics, as an advantageous approach in prevention and treatment of IBD.
ISSN:0378-5173
1873-3476
DOI:10.1016/j.ijpharm.2017.05.049