Overexpression of StNF-YB3.1 reduces photosynthetic capacity and tuber production, and promotes ABA-mediated stomatal closure in potato (Solanum tuberosum L.)

• Published in: Plant science (Limerick) 2017-08, Vol.261, p.50-59
• Main Authors: Xuanyuan, Guochao, Lu, Congming, Zhang, Ruofang, Jiang, Jiming
• Format: Article
• Language: English
• Subjects: ABA-mediated stomatal closure; Abscisic Acid - physiology; Chlorophyll - metabolism; Gene Expression Regulation, Plant - genetics; Gene Expression Regulation, Plant - physiology; Genes, Plant - genetics; Genes, Plant - physiology; NF-Y; Photosynthesis - physiology; Photosynthetic rate; Plant Growth Regulators - physiology; Plant Proteins - metabolism; Plant Proteins - physiology; Plant Stomata - physiology; Plant Tubers - growth & development; Potato; Solanum tuberosum - growth & development; Solanum tuberosum - metabolism; Solanum tuberosum - physiology; Stomatal conductance; Transcription Factors - metabolism; Transcription Factors - physiology
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2017.04.015

•StNF-YB3.1, the first potato gene encoding a subunit of the NF-Y transcription factor, was identified and characterized.•Overexpression of StNF-YB3.1 results in acceleration of the onset of flowering and significant increase in leaf chlorophyll content in transgenic potato plants.•Transgenic potato plants overexpressing StNF-YB3.1 display decreased photosynthetic capacity and reduced tuber production.•Overexpression of StNF-YB3.1 up-regulates the expression of genes involved in ABA signaling pathway and enhances ABA-mediated stomatal closure. Nuclear factor Y (NF-Y) is one of the most ubiquitous transcription factors (TFs), comprising NF-YA, NF-YB and NF-YC subunits, and has been identified and reported in various aspects of development for plants and animals. In this work, StNF-YB3.1, a putative potato NF-YB subunit encoding gene, was isolated from Solanum tuberosum by rapid amplification of cDNA ends (RACE). Overexpression of StNF-YB3.1 in potato (cv. Atlantic) resulted in accelerated onset of flowering, and significant increase in leaf chlorophyll content in field trials. However, transgenic potato plants overexpressing StNF-YB3.1 (OEYB3.1) showed significant decreases in photosynthetic rate and stomatal conductance both at tuber initiation and bulking stages. OEYB3.1 lines were associated with significantly fewer tuber numbers and yield reduction. Guard cell size and stomatal density were not changed in OEYB3.1 plants, whereas ABA-mediated stomatal closure was accelerated compared to that of wild type plants because of the up-regulation of genes for ABA signaling, such as StCPK10-like, StSnRK2.6/OST1-like, StSnRK2.7-like and StSLAC1-like. We speculate that the acceleration of stomatal closure was a possible reason for the significantly decreased stomatal conductance and photosynthetic rate.