Construction of Injectable Double-Network Hydrogels for Cell Delivery

Herein we present a unique method of using dynamic cross-links, which are dynamic covalent bonding and ionic interaction, for the construction of injectable double-network (DN) hydrogels, with the objective of cell delivery for cartilage repair. Glycol chitosan and dibenzaldhyde capped poly­(ethylen...

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Bibliographic Details
Published in:Biomacromolecules 2017-07, Vol.18 (7), p.2128-2138
Main Authors: Yan, Yan, Li, Mengnan, Yang, Di, Wang, Qian, Liang, Fuxin, Qu, Xiaozhong, Qiu, Dong, Yang, Zhenzhong
Format: Article
Language:English
Subjects:
Animals
Cell Line
Cell Transplantation - methods
Cells, Immobilized - metabolism
Cells, Immobilized - transplantation
Chondrogenesis
Hydrogels - chemical synthesis
Hydrogels - chemistry
Hydrogels - pharmacology
Male
Mice
Mice, Inbred BALB C
Polyethylene Glycols - chemistry
Polyethylene Glycols - pharmacology
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Summary:Herein we present a unique method of using dynamic cross-links, which are dynamic covalent bonding and ionic interaction, for the construction of injectable double-network (DN) hydrogels, with the objective of cell delivery for cartilage repair. Glycol chitosan and dibenzaldhyde capped poly­(ethylene oxide) formed the first network, while calcium alginate formed the second one, and in the resultant DN hydrogel, either of the networks could be selectively removed. The moduli of the DN hydrogel were significantly improved compared to that of the parent single-network hydrogels and were tunable by changing the chemical components. In situ 3D cell encapsulation could be easily performed by mixing cell suspension to the polymer solutions and transferred through a syringe needle before sol–gel transition. Cell proliferation and mediated differentiation of mouse chondrogenic cells were achieved in the DN hydrogel extracellular matrix.
ISSN:1525-7797
1526-4602
DOI:10.1021/acs.biomac.7b00452