Biosynthesis of Silver Nanoparticles by Streptomyces griseorubens isolated from Soil and Their Antioxidant Activity

• Published in: IET nanobiotechnology 2017-04, Vol.11 (3), p.286-291
• Main Authors: Baygar, Tuba, Ugur, Aysel
• Format: Article
• Language: English
• Subjects: 2,2‐diphenyl‐1‐picrylhydrazyl free radical scavenging assay; absorption coefficients; Absorption spectra; active biological components; antioxidant activity; Antioxidants; Antioxidants - chemistry; Biological Products - administration & dosage; Biological Products - chemistry; Biosynthesis; cellular biophysics; cell‐free culture supernatant; crystalline particles; DNA; Dose-Response Relationship, Drug; elemental analysis; energy dispersive X‐ray spectroscopy; Fourier transform infrared spectra; Fourier transform infrared spectroscopy; Free Radical Scavengers - chemistry; Free radicals; high‐resolution transmission electron microscopy; Materials Testing; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; microbial mediated biological synthesis; Microorganisms; molecular biophysics; molecular characterisation method; nanofabrication; Nanoparticles; Nanostructure; Particle Size; rDNA sequencing; Research Article; scanning electron microscopy; Silver; Silver - chemistry; silver nanoparticles; size 5 nm to 20 nm; soil; Soil Microbiology; spherical particles; strain identification; Streptomyces; Streptomyces griseorubens AU2; Streptomyces griseus - metabolism; transmission electron microscopy; ultraviolet spectra; ultraviolet‐visible spectroscopy; UV‐vis spectroscopy; visible spectra; wavelength 422 nm; X‐ray chemical analysis; X‐ray diffraction
• ISSN: 1751-8741; 1751-875X; 1751-875X
• DOI: 10.1049/iet-nbt.2015.0127

Microbial mediated biological synthesis of metallic nanoparticles was carried out ecofriendly in the present study. Silver nanoparticles (AgNPs) were extracellularly biosynthesised from Streptomyces griseorubens AU2 and extensively characterised by ultraviolet–visible (UV–vis) and Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy, scanning electron microscopy and X-ray diffraction analysis. Elemental analysis of nanoparticles was also carried out using energy dispersive X-ray spectroscopy. The biosynthesised AgNPs showed the characteristic absorption spectra in UV–vis at 422 nm which confirmed the presence of metallic AgNPs. According to the further characterisation analysis, the biosynthesised AgNPs were found to be spherical and crystalline particles with 5–20 nm average size. Antioxidant properties of the biosynthesised AgNPs were determined by 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay and was found to increase in a dose-dependent matter. The identification of the strain was determined by molecular characterisation method using 16s rDNA sequencing. The present study is the first report on the microbial biosynthesis of AgNPs using S. griseorubens isolated from soil and provides that the active biological components found in the cell-free culture supernatant of S. griseorubens AU2 enable the synthesis of AgNPs.