The convergent chemical synthesis of histone H3 protein for site-specific acetylation at Lys56 and ubiquitination at Lys122

Deposition of (H3-H4) tetramers is believed to be the critical step in nucleosome assembly. Site-specific acetylation and ubiquitination of histone H3 have been speculated to synergistically facilitate the formation and deposition of (H3-H4) tetramers. Here we report our endeavors toward the first c...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2017, Vol.53 (29), p.4148-4151
Main Authors: Qi, Yun-Kun, He, Qiao-Qiao, Ai, Hua-Song, Guo, Jing, Li, Jia-Bin
Format: Article
Language:English
Subjects:
Acetylation
Assembly
Biochemistry
Deposition
Formations
Histones
Histones - chemical synthesis
Histones - chemistry
Histones - metabolism
Lysine - chemistry
Lysine - metabolism
Proteins
Synthesis (chemistry)
Ubiquitination
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Summary:Deposition of (H3-H4) tetramers is believed to be the critical step in nucleosome assembly. Site-specific acetylation and ubiquitination of histone H3 have been speculated to synergistically facilitate the formation and deposition of (H3-H4) tetramers. Here we report our endeavors toward the first chemical synthesis of homogenous histone H3, which bears Lys56 acetylation and Lys122 ubiquitination, for in vitro biochemical and biophysical studies.
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc01721a