Effect of chromium (VI) on the multiple nitrogen removal pathways and microbial community of aerobic granular sludge

• Published in: Environmental technology 2018-07, Vol.39 (13), p.1682-1696
• Main Authors: Zheng, Xiao-Ying, Lu, Dan, Wang, Ming-Yang, Chen, Wei, Zhou, Gan, Zhang, Yuan
• Format: Article
• Language: English
• Subjects: Ammonia; Ammonia-oxidizing bacteria; Autotrophic bacteria; Bacteria; Chromium; Communities; Denitrification; Denitrifying bacteria; Dominant species; Electrophoresis; Gel electrophoresis; Heterotrophic bacteria; Inhibition; Long-term effects; Metabolism; Microbiomes; Microorganisms; Nitrification; Nitrogen; Nitrogen removal; Oxidation; Sludge; Soil microorganisms; Wastewater
• ISSN: 0959-3330; 1479-487X
• DOI: 10.1080/09593330.2017.1337230

The frequent appearance of Cr(VI) significantly impacts the microbial metabolism in wastewater. In this study, long-term effects of Cr(VI) on microbial community, nitrogen removal pathways and mechanism of aerobic granular sludge (AGS) were investigated. AGS had strong resistance ability to 1.0 mg/L Cr(VI). 3.0 mg/L Cr(VI) increased the heterotrophic-specific ammonia uptake rate (HSAUR) and heterotrophic-specific nitrate uptake rate (HSNUR) transiently, whereas 5.0 mg/L Cr(VI) sharply decreased the specific ammonia uptake rate (SAUR), specific nitrate uptake rate (SNUR) and simultaneous nitrification denitrification rate (SNDR). It was found that Cr (VI) has a greater inhibitory effect on autotrophic nitrification (ASAUR), and the maximal inhibition rate (IR) was 139.19%. Besides, the inhibition of Cr (VI) on nitrogen removal process belongs to non-competitive inhibition. Cr(VI) had a weaker negative impact on heterotrophic bacteria compared with that on autotrophic bacteria. Denaturing gradient gel electrophoresis analyses suggest that Acidovorax sp., flavobacterium sp., uncultured soil bacterium, uncultured nitrosospira sp., uncultured prokaryote, uncultured β-proteobacterium and uncultured pseudomonas sp. were the dominant species. The inhibition of Cr(VI) on nitrite-oxidizing bacteria was the strongest, followed by ammonia-oxidizing bacteria and denitrifying bacteria. Linear correlations between bacterial count and biomass-specific uptake rate were observed when the Cr(VI) concentration exceeded 3 mg/L. This study revealed the effect of Cr(VI) on nitrification is more serious than that on denitrification. Autotrophic and heterotrophic nitrification, heterotrophic denitrification and simultaneous nitrification denitrification played a significant role on nitrogen removal under Cr(VI) stress.