Size-exclusion chromatography of xylan derivatives—the critical evaluation of macromolecular data

Hydroxypropyl xylans with varying degrees of substitution were characterized by size-exclusion chromatography. Molar masses of the samples were determined using two approaches: by conventional calibration with molar mass standards and by a multi-detection method that utilizes the combination of stat...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2017-08, Vol.409 (20), p.4811-4817
Main Authors: Pitkänen, Leena, Aseyev, Vladimir, Laine, Christiane, Tuomainen, Päivi, Tenkanen, Maija
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biochemistry
Calibration
Chains (polymeric)
Characterization and Evaluation of Materials
Chemical properties
Chemistry
Chemistry and Materials Science
Chromatography
Conformation
Food Science
Gel permeation chromatography
Laboratory Medicine
Light scattering
Macromolecules
Methods
Molecular conformation
Monitoring/Environmental Analysis
Polymers
Pullulan
Refractivity
Research Paper
Size exclusion chromatography
Viscometry
Xylan
Xylans
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Summary:Hydroxypropyl xylans with varying degrees of substitution were characterized by size-exclusion chromatography. Molar masses of the samples were determined using two approaches: by conventional calibration with molar mass standards and by a multi-detection method that utilizes the combination of static light scattering, viscometry, and differential refractive index detection. The molar mass results obtained by the multi-detection method were accurate, but required the determination of separate refractive index increments for each structurally different sample. The column calibration approach with standard pullulan samples gave biased results due to the differences in hydrodynamic volumes between pullulans and hydroxypropyl xylans with similar molar masses. The degree of hydroxypropylation affected the chain conformation and compactness of the polymer chains. Mark-Houwink parameters and persistence length values suggested that the hydroxypropyl substituents reduced the flexibility of the xylan chain and made the polymer chain more extended.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-017-0424-5