Initial Stages of Angiogenesis after Acute Experimental Local Venous Outflow Disturbances and Application of Cell Technologies

The initial stages of angiogenesis in rats after transcutaneous injection of autologous bone marrow multipotent mesenchymal stromal cells transfected with GFP gene and stained cell membranes in the projection of ligated femoral vein were studied by fluorescent light and confocal laser microscopy. La...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2017-05, Vol.163 (1), p.142-147
Main Authors: Maiborodin, I. V., Morozov, V. V., Matveeva, V. A., Anikeev, A. A., Maslov, R. V., Chastikin, G. A., Figurenko, N. F.
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Autografts
Biomedical and Life Sciences
Biomedicine
Bone marrow
Cell Biology
Cell membranes
Cells, Cultured
Femoral Vein - cytology
Femoral Vein - metabolism
Femur
Green fluorescent protein
Internal Medicine
Laboratory Medicine
Lymph nodes
Lymph Nodes - cytology
Lymph Nodes - metabolism
Macrophages
Male
Mesenchymal Stem Cell Transplantation
Mesenchymal stem cells
Mesenchymal Stromal Cells - metabolism
Mesenchymal Stromal Cells - physiology
Mesenchyme
Neovascularization, Physiologic - physiology
Parenchyma
Pathology
Rats
Stem cells
Stromal cells
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
Veins - cytology
Veins - metabolism
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Description
Summary:The initial stages of angiogenesis in rats after transcutaneous injection of autologous bone marrow multipotent mesenchymal stromal cells transfected with GFP gene and stained cell membranes in the projection of ligated femoral vein were studied by fluorescent light and confocal laser microscopy. Large clusters of brightly fluorescing elongated fibroblast-like cells were seen in the paravasal tissue and in the postoperative scar and signs of angiogenesis were noted as soon as in 4 days. The injected cells not only formed new vessels, but also integrated into vessels formed by host cells. Some injected cells were phagocytizied by macrophages and the latter started to fluoresce due to the presence of the membrane dye. These macrophages within the specified period appeared in the regional inguinal lymph nodes where they formed clusters in the lymphoid parenchyma of the cortical substance.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-017-3755-5