Efficient fluorescence detection of protoporphyrin IX in metastatic lymph nodes of murine colorectal cancer stained with indigo carmine

• Published in: Photodiagnosis and photodynamic therapy 2017-09, Vol.19, p.175-180
• Main Authors: Matsuo, Hisataka, Harada, Yoshinori, Minamikawa, Takeo, Kato, Yoshiyuki, Murayama, Yasutoshi, Otsuji, Eigo, Takamatsu, Tetsuro, Tanaka, Hideo
• Format: Article
• Language: English
• Subjects: 5-Aminolevulinic acid; Animals; Colorectal Neoplasms - pathology; Coloring Agents - pharmacokinetics; Coloring Agents - pharmacology; Hematology, Oncology and Palliative Medicine; Indigo carmine; Indigo Carmine - pharmacokinetics; Indigo Carmine - pharmacology; Internal Medicine; Lymph node metastasis; Lymph Nodes - diagnostic imaging; Mice; Neoplasm Metastasis; Optical Imaging - methods; Protoporphyrin IX; Protoporphyrins - pharmacokinetics; Protoporphyrins - pharmacology
• ISSN: 1572-1000; 1873-1597
• DOI: 10.1016/j.pdpdt.2017.05.019

Highlights • The diagnostic usefulness of protoporphyrin IX (PpIX) fluorescence was evaluated in the presence of indigo carmine in a mouse lymph node (LN) metastasis model of rectal cancer after administration of 5-aminolevulinc acid (5-ALA). • Indigo carmine is a blue dye commonly used for identification of sentinel LNs during surgery. • The absorption spectrum of indigo carmine widely overlapped with the fluorescence spectrum of PpIX at the peak of 632 nm, but not at the other peak of 700 nm. • Fluorescence measurement was more efficient at 700 nm than at 632 nm for detection of PpIX in metastatic LNs stained with indigo carmine.