Two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus

Abstract Exfoliative toxin B (ETB) encoded by some large plasmids plays a crucial role in epidermolytic diseases caused by Staphylococcus aureus . We have found as yet unknown types of etb gene-positive plasmids isolated from a set of impetigo strains implicated in outbreaks of pemphigus neonatorum...

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Bibliographic Details
Published in:International journal of medical microbiology 2017-09, Vol.307 (6), p.291-296
Main Authors: Botka, Tibor, Růžičková, Vladislava, Svobodová, Karla, Pantůček, Roman, Petráš, Petr, Čejková, Darina, Doškař, Jiří
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacteriocins - genetics
Bullous impetigo
Czech Republic - epidemiology
Dermotoxins - genetics
DNA, Bacterial - genetics
ETB plasmids
Exfoliatins - genetics
Exfoliative toxin B
Humans
Impetigo - epidemiology
Impetigo - microbiology
Infectious Disease
Medical Education
Pemphigus - epidemiology
Pemphigus - microbiology
Phylogeny
Plasmids - genetics
Plasmids - isolation & purification
Staphylococcus aureus
Staphylococcus aureus - classification
Staphylococcus aureus - genetics
Virulence - genetics
Whole Genome Sequencing
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Summary:Abstract Exfoliative toxin B (ETB) encoded by some large plasmids plays a crucial role in epidermolytic diseases caused by Staphylococcus aureus . We have found as yet unknown types of etb gene-positive plasmids isolated from a set of impetigo strains implicated in outbreaks of pemphigus neonatorum in Czech maternity hospitals. Plasmids from the strains of clonal complex CC121 were related to archetypal plasmid pETBTY4 . Sharing a 33-kb core sequence including virulence genes for ETB, EDIN C, and lantibiotics, they were assigned to a stand-alone lineage, named pETBTY4 -based plasmids. Differing from each other in the content of variable DNA regions, they formed four sequence types. In addition to them, a novel unique plasmid pETB608 isolated from a strain of ST130 was described. Carrying conjugative cluster genes, as well as new variants of etb and edinA genes, pETB608 could be regarded as a source of a new lineage of ETB plasmids. We have designed a helpful detection assay, which facilitates the precise identification of the all described types of ETB plasmids.
ISSN:1438-4221
1618-0607
DOI:10.1016/j.ijmm.2017.05.005