The regulation of SKF38393 on the signaling pathway of dopamine D1 receptor in hippocampus during chronic sleep deprivation

•CSD decreased the expression of CREB in hippocampus of rats by D1R’s signaling pathways.•PKA and CaMK IV were crucial molecules during the improvement of hippocampal function.•SKF38393 might improve the CREB in CSD rats through PKA and phosphoinositide pathway. Hippocampal functions are sensitive t...

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Bibliographic Details
Published in:Neuroscience letters 2017-07, Vol.654, p.42-48
Main Authors: Chen, Si, Wen, Xiaosa, Liang, Han, Li, Yuxiang, Chen, Xinmin, Zhang, Dong, Shi, Rengfei, Ma, Wenling
Format: Article
Language:English
Subjects:
Chronic sleep deprivation
Dopamine D1 receptor
Hippocampus
Signaling pathway
SKF38393
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Summary:•CSD decreased the expression of CREB in hippocampus of rats by D1R’s signaling pathways.•PKA and CaMK IV were crucial molecules during the improvement of hippocampal function.•SKF38393 might improve the CREB in CSD rats through PKA and phosphoinositide pathway. Hippocampal functions are sensitive to sleep deficiency. Dopamine D1 receptor (D1R) in hippocampus can regulate the expression of cAMP response element binding protein (CREB) through PKA, MAPK and phosphoinositide pathway, but which pathway plays the major role in hippocampus during Chronic sleep deprivation (CSD) is unclear. The CSD model was created, SKF rats were administered the D1R agonist (SKF38363), and hippocampus from each animal was dissected for following molecular detection. The gene and protein levels of CREB and key molecules in D1R pathways were measured by real-time PCR and western blotting, respectively. Both the gene and protein expression of CREB in hippocampus decreased by CSD and improved significantly by SKF38393 (p
ISSN:0304-3940
1872-7972
DOI:10.1016/j.neulet.2017.05.072