Effect of carrageenans alone and in combination with casein or lipopolysaccharide on human epithelial intestinal HT‐29 cells

• Published in: Journal of biomedical materials research. Part A 2017-10, Vol.105 (10), p.2843-2850
• Main Authors: Sokolova, E. V., Kuz'mich, A. S., Byankina, A. O., Yermak, I. M.
• Format: Article
• Language: English
• Subjects: Algae; Animals; Binding; Carrageenan; Carrageenan - chemistry; Carrageenan - pharmacology; Carrageenans; Casein; Caseins - chemistry; Caseins - pharmacology; Cell culture; Cell Survival - drug effects; Escherichia coli - chemistry; Ethanol; Exposure; HT29 Cells; HT‐29; Humans; Intestinal Mucosa - cytology; Intestinal Mucosa - drug effects; Intestine; Large intestine; lipopolysaccharide; Lipopolysaccharides; Lipopolysaccharides - chemistry; Lipopolysaccharides - pharmacology; Milk - chemistry; Monolayers; Mucin; Polysaccharides; protein binding; Rhodophyta - chemistry; Saccharides; Small intestine; Stress; Stresses; Sulfate; Toluidine; Toluidine blue; xCelligence
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.36136

The research described here was focused on the effect on human intestinal epithelial cell monolayers of sulfated red algal polysaccharides (κ‐, λ‐, and κ/β‐carrageenans) alone and in combination with casein or lipopolysaccharide (LPS). HT‐29 cells were investigated under normal and stress conditions; stress was induced by exposure to ethanol. Cell viability was monitored with a real‐time system. The change in binding properties of negatively sulfated red algal polysaccharides assessed by the measurement of free carrageenans in mixtures with casein or McCoy's 5 A culture medium by means of toluidine blue O. Low sulfate content and the presence of 3,6‐anhydogalactose are prerequisites for the recovery of ethanol‐exposed HT‐29 cells by carrageenans. Analysis of carrageenan binding ability confirmed that casein and LPS should affect carrageenan activity. Whether the combined action of the mucin‐containing layer and carrageenans or the action of carrageenans alone was responsible for enhanced cell viability under stress conditions induced by ethanol is a subject for further research. © 2017 Wiley Periodicals Inc. J Biomed Mater Res Part A: 105A: 2843–2850, 2017.