Near infrared fluorescent dual ligand functionalized Au NCs based multidimensional sensor array for pattern recognition of multiple proteins and serum discrimination

• Published in: Biosensors & bioelectronics 2017-11, Vol.97, p.203-207
• Main Authors: Xu, Shenghao, Gao, Teng, Feng, Xiuying, Fan, Xiaojian, Liu, Gufan, Mao, Yaning, Yu, Xijuan, Lin, Jiehua, Luo, Xiliang
• Format: Article
• Language: English
• Subjects: Amino Acids - chemistry; Biosensing Techniques - methods; Blood Proteins - analysis; Breast Neoplasms - blood; Calorimetry - methods; Cancer serum discrimination; Cross-reactive sensor array; Discriminant Analysis; Dual ligand functionalized Au NCs; Female; Fluorescent Dyes - chemistry; Gold - chemistry; Humans; Limit of Detection; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; Nanostructures - chemistry; Nanostructures - ultrastructure; Pattern recognition; Protein discrimination
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2017.06.007

Here, a multidimensional sensor array capable of analyzing various proteins and discriminating between serums from different stages of breast cancer patients were developed based on six kinds of near infrared fluorescent dual ligand functionalized Au NCs (functionalized with different amino acids) as sensing receptors. These six kinds of different amino acids functionalized Au NCs were synthesized for the first time within 2h due to the direct donation of delocalized electrons of electron-rich atoms or groups of the ligands to the Au core. Based on this, ten proteins could be simultaneously and effectively discriminated by this “chemical nose/tongue” sensor array. Linear discrimination analysis (LDA) of the response patterns showed successful differentiation of the analytes at concentrations as low as 10nM with high identification accuracy. Isothermal titration calorimetry (ITC) experiment illustrates that Au NCs interacted with proteins mainly by hydrogen bonding and van der Waals forces. Furthermore, the greatest highlight of this sensor array is demonstrated by successfully discriminating between serums from different stages of breast cancer patients (early, middle and late) and healthy people, suggesting great potential for auxiliary diagnosis. •Serums from different stages of breast cancer patients and healthy people can be discriminated.•Au nanoclusters functionalized with different amino acids were utilized as sensing receptors.•This sensor array is sufficiently sensitive to assay proteins at nanomolar concentrations.