In situ monitoring of molecular changes during cell differentiation processes in marine macroalgae through mass spectrometric imaging

Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) was employed to discriminate between cell differentiation processes in macroalgae. One of the key developmental processes in the algal life cycle is the production of germ cells (gametes and zoids). The gametogenesis...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2017-08, Vol.409 (20), p.4893-4903
Main Authors: Kessler, Ralf W., Crecelius, Anna C., Schubert, Ulrich S., Wichard, Thomas
Format: Article
Language:English
Subjects:
Algae
Analytical Chemistry
Biochemistry
Biomarkers
Cell differentiation
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Data analysis
Data processing
Desorption
Differentiation (biology)
Dimethylsulphoniopropionate
Food Science
Gametangia
Gametes
Gametogenesis
Germ cells
Ionization
Ions
Laboratory Medicine
Lasers
Life cycle engineering
Life cycles
Mass spectrometry
Metabolomics
Methods
Molecular modelling
Monitoring/Environmental Analysis
Observations
Physiological aspects
Research Paper
Seaweeds
Spectrometry
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Summary:Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) was employed to discriminate between cell differentiation processes in macroalgae. One of the key developmental processes in the algal life cycle is the production of germ cells (gametes and zoids). The gametogenesis of the marine green macroalga Ulva mutabilis (Chlorophyta) was monitored by metabolomic snapshots of the surface, when blade cells differentiate synchronously into gametangia and giving rise to gametes. To establish MSI for macroalgae, dimethylsulfoniopropionate (DMSP), a known algal osmolyte, was determined. MSI of the surface of U. mutabilis followed by chemometric data analysis revealed dynamic metabolomic changes during cell differentiation. DMSP and a total of 55 specific molecular biomarkers, which could be assigned to important stages of the gametogenesis, were detected. Our research contributes to the understanding of molecular mechanisms underlying macroalgal cell differentiation. Graphical abstract Molecular changes during cell differentiation of the marine macroalga Ulva were visualized by matrix assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI)
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-017-0430-7