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In situ monitoring of molecular changes during cell differentiation processes in marine macroalgae through mass spectrometric imaging
Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) was employed to discriminate between cell differentiation processes in macroalgae. One of the key developmental processes in the algal life cycle is the production of germ cells (gametes and zoids). The gametogenesis...
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| Published in: | Analytical and bioanalytical chemistry 2017-08, Vol.409 (20), p.4893-4903 |
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| cites | cdi_FETCH-LOGICAL-c448t-e52a7090bc72b56665ff4c62f42e83631e6a4427b86a5f508c878118712bd56b3 |
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| container_title | Analytical and bioanalytical chemistry |
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| creator | Kessler, Ralf W. Crecelius, Anna C. Schubert, Ulrich S. Wichard, Thomas |
| description | Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) was employed to discriminate between cell differentiation processes in macroalgae. One of the key developmental processes in the algal life cycle is the production of germ cells (gametes and zoids). The gametogenesis of the marine green macroalga
Ulva mutabilis
(Chlorophyta) was monitored by metabolomic snapshots of the surface, when blade cells differentiate synchronously into gametangia and giving rise to gametes. To establish MSI for macroalgae, dimethylsulfoniopropionate (DMSP), a known algal osmolyte, was determined. MSI of the surface of
U. mutabilis
followed by chemometric data analysis revealed dynamic metabolomic changes during cell differentiation. DMSP and a total of 55 specific molecular biomarkers, which could be assigned to important stages of the gametogenesis, were detected. Our research contributes to the understanding of molecular mechanisms underlying macroalgal cell differentiation.
Graphical abstract
Molecular changes during cell differentiation of the marine macroalga
Ulva
were visualized by matrix assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) |
| doi_str_mv | 10.1007/s00216-017-0430-7 |
| format | article |
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Ulva mutabilis
(Chlorophyta) was monitored by metabolomic snapshots of the surface, when blade cells differentiate synchronously into gametangia and giving rise to gametes. To establish MSI for macroalgae, dimethylsulfoniopropionate (DMSP), a known algal osmolyte, was determined. MSI of the surface of
U. mutabilis
followed by chemometric data analysis revealed dynamic metabolomic changes during cell differentiation. DMSP and a total of 55 specific molecular biomarkers, which could be assigned to important stages of the gametogenesis, were detected. Our research contributes to the understanding of molecular mechanisms underlying macroalgal cell differentiation.
Graphical abstract
Molecular changes during cell differentiation of the marine macroalga
Ulva
were visualized by matrix assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI)</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-017-0430-7</identifier><identifier>PMID: 28600691</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Algae ; Analytical Chemistry ; Biochemistry ; Biomarkers ; Cell differentiation ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Data analysis ; Data processing ; Desorption ; Differentiation (biology) ; Dimethylsulphoniopropionate ; Food Science ; Gametangia ; Gametes ; Gametogenesis ; Germ cells ; Ionization ; Ions ; Laboratory Medicine ; Lasers ; Life cycle engineering ; Life cycles ; Mass spectrometry ; Metabolomics ; Methods ; Molecular modelling ; Monitoring/Environmental Analysis ; Observations ; Physiological aspects ; Research Paper ; Seaweeds ; Spectrometry</subject><ispartof>Analytical and bioanalytical chemistry, 2017-08, Vol.409 (20), p.4893-4903</ispartof><rights>Springer-Verlag Berlin Heidelberg 2017</rights><rights>COPYRIGHT 2017 Springer</rights><rights>Analytical and Bioanalytical Chemistry is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-e52a7090bc72b56665ff4c62f42e83631e6a4427b86a5f508c878118712bd56b3</citedby><cites>FETCH-LOGICAL-c448t-e52a7090bc72b56665ff4c62f42e83631e6a4427b86a5f508c878118712bd56b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28600691$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kessler, Ralf W.</creatorcontrib><creatorcontrib>Crecelius, Anna C.</creatorcontrib><creatorcontrib>Schubert, Ulrich S.</creatorcontrib><creatorcontrib>Wichard, Thomas</creatorcontrib><title>In situ monitoring of molecular changes during cell differentiation processes in marine macroalgae through mass spectrometric imaging</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) was employed to discriminate between cell differentiation processes in macroalgae. One of the key developmental processes in the algal life cycle is the production of germ cells (gametes and zoids). The gametogenesis of the marine green macroalga
Ulva mutabilis
(Chlorophyta) was monitored by metabolomic snapshots of the surface, when blade cells differentiate synchronously into gametangia and giving rise to gametes. To establish MSI for macroalgae, dimethylsulfoniopropionate (DMSP), a known algal osmolyte, was determined. MSI of the surface of
U. mutabilis
followed by chemometric data analysis revealed dynamic metabolomic changes during cell differentiation. DMSP and a total of 55 specific molecular biomarkers, which could be assigned to important stages of the gametogenesis, were detected. Our research contributes to the understanding of molecular mechanisms underlying macroalgal cell differentiation.
Graphical abstract
Molecular changes during cell differentiation of the marine macroalga
Ulva
were visualized by matrix assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI)</description><subject>Algae</subject><subject>Analytical Chemistry</subject><subject>Biochemistry</subject><subject>Biomarkers</subject><subject>Cell differentiation</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Data analysis</subject><subject>Data processing</subject><subject>Desorption</subject><subject>Differentiation (biology)</subject><subject>Dimethylsulphoniopropionate</subject><subject>Food Science</subject><subject>Gametangia</subject><subject>Gametes</subject><subject>Gametogenesis</subject><subject>Germ cells</subject><subject>Ionization</subject><subject>Ions</subject><subject>Laboratory Medicine</subject><subject>Lasers</subject><subject>Life cycle engineering</subject><subject>Life cycles</subject><subject>Mass spectrometry</subject><subject>Metabolomics</subject><subject>Methods</subject><subject>Molecular modelling</subject><subject>Monitoring/Environmental Analysis</subject><subject>Observations</subject><subject>Physiological aspects</subject><subject>Research 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situ monitoring of molecular changes during cell differentiation processes in marine macroalgae through mass spectrometric imaging</title><author>Kessler, Ralf W. ; Crecelius, Anna C. ; Schubert, Ulrich S. ; Wichard, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-e52a7090bc72b56665ff4c62f42e83631e6a4427b86a5f508c878118712bd56b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Algae</topic><topic>Analytical Chemistry</topic><topic>Biochemistry</topic><topic>Biomarkers</topic><topic>Cell differentiation</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Data analysis</topic><topic>Data processing</topic><topic>Desorption</topic><topic>Differentiation (biology)</topic><topic>Dimethylsulphoniopropionate</topic><topic>Food 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chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2017-08-01</date><risdate>2017</risdate><volume>409</volume><issue>20</issue><spage>4893</spage><epage>4903</epage><pages>4893-4903</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) was employed to discriminate between cell differentiation processes in macroalgae. One of the key developmental processes in the algal life cycle is the production of germ cells (gametes and zoids). The gametogenesis of the marine green macroalga
Ulva mutabilis
(Chlorophyta) was monitored by metabolomic snapshots of the surface, when blade cells differentiate synchronously into gametangia and giving rise to gametes. To establish MSI for macroalgae, dimethylsulfoniopropionate (DMSP), a known algal osmolyte, was determined. MSI of the surface of
U. mutabilis
followed by chemometric data analysis revealed dynamic metabolomic changes during cell differentiation. DMSP and a total of 55 specific molecular biomarkers, which could be assigned to important stages of the gametogenesis, were detected. Our research contributes to the understanding of molecular mechanisms underlying macroalgal cell differentiation.
Graphical abstract
Molecular changes during cell differentiation of the marine macroalga
Ulva
were visualized by matrix assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI)</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28600691</pmid><doi>10.1007/s00216-017-0430-7</doi><tpages>11</tpages></addata></record> |
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| language | eng |
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| subjects | Algae Analytical Chemistry Biochemistry Biomarkers Cell differentiation Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Data analysis Data processing Desorption Differentiation (biology) Dimethylsulphoniopropionate Food Science Gametangia Gametes Gametogenesis Germ cells Ionization Ions Laboratory Medicine Lasers Life cycle engineering Life cycles Mass spectrometry Metabolomics Methods Molecular modelling Monitoring/Environmental Analysis Observations Physiological aspects Research Paper Seaweeds Spectrometry |
| title | In situ monitoring of molecular changes during cell differentiation processes in marine macroalgae through mass spectrometric imaging |
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