Antioxidant flavone analog functionalized fluorescent silica nanoparticles: Synthesis and exploration of their possible use as biomolecule sensor

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2017-09, Vol.157, p.286-296
• Main Authors: Das, Sreeparna, Batuta, Shaikh, Alam, Md. Niharul, Fouzder, Chandrani, Kundu, Rakesh, Mandal, Debabrata, Begum, Naznin Ara
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant flavone analog; Antioxidants - chemistry; Biocompatible; Biomolecule sensor; Biosensing Techniques - methods; BSA; Cattle; Cell Line, Tumor; Flavones - chemistry; Fluorescent silica nanoparticles; Humans; Nanoparticles - chemistry; Serum Albumin, Bovine - chemistry; Silicon Dioxide - chemistry
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2017.05.074

[Display omitted] •Antioxidant flavone modified fluorescent silica nanoparticles (NPs) synthesis.•Anchoring of a fluorescent synthetic antioxidant flavone analog on NP surface.•Mesoporous silica NP matrix is tagged with flavone through sulfonamide linkage.•These NPs are biocompatible, retain their intrinsic fluorescence in cellular medium.•These fluorescent NPs can have the potential to probe biomolecules, like BSA. For the first time, a synthetic fluorescent antioxidant flavone analog was successfully anchored onto the surface of the APTES-modified mesoporous silica nanoparticles (NPs) through sulfonamide linkage. The surface chemistry and morphology of the flavone modified fluorescent silica (FMFS) NPs were studied in detail. The flavone moiety when attached onto the FMFS NP surface, imparted its characteristic fluorescence and antioxidant activities to these NPs. Moreover, the NPs are highly biocompatible as evidenced from their cytotoxicity assay on normal lung cell (L132). The fluorescence activity of these biocompatible NPs was further utilized to study their interaction with a biomolecule, BSA (Bovine Serum Albumin). It was interesting to note that the fluorescence behavior of FMFS NPs completely changed on their binding with BSA. On the other hand, the intrinsic fluorescence activity of BSA was also significantly modified due to its interaction with FMFS NPs. Thus, the sensing and detection of biomolecules like BSA in presence of FMFS NPs can be accomplished by monitoring changes in the fluorescence behavior of either FMFS NPs or BSA. Furthermore, these FMFS NPs retained their intrinsic fluorescence behavior in the cellular medium which opens up their possible use as biocompatible cell imaging agents in future.