Analysis of Lysophospholipid Content in Low Phytate Rice Mutants

As a fundamental component of nucleic acids, phospholipids, and adenosine triphosphate, phosphorus (P) is critical to all life forms, however, the molecular mechanism of P translocation and distribution in rice grains are still not understood. Here, with the use of five different low phytic acid (lp...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2017-07, Vol.65 (26), p.5435-5441
Main Authors: Tong, Chuan, Chen, Yaling, Tan, Yuanyuan, Liu, Lei, Waters, Daniel L. E, Rose, Terry J, Shu, Qingyao, Bao, Jinsong
Format: Article
Language:English
Subjects:
Lysophospholipids - analysis
Lysophospholipids - metabolism
Mutation
Oryza - chemistry
Oryza - genetics
Oryza - metabolism
Phosphorus - analysis
Phosphorus - metabolism
Phytic Acid - analysis
Phytic Acid - metabolism
Seeds - chemistry
Seeds - genetics
Seeds - metabolism
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Summary:As a fundamental component of nucleic acids, phospholipids, and adenosine triphosphate, phosphorus (P) is critical to all life forms, however, the molecular mechanism of P translocation and distribution in rice grains are still not understood. Here, with the use of five different low phytic acid (lpa) rice mutants, the redistribution in the main P-containing compounds in rice grain, phytic acid (PA), lysophospholipid (LPL), and inorganic P (Pi), was investigated. The lpa mutants showed a significant decrease in PA and phytate-phosphorus (PA-P) concentration with a concomitant increase in Pi concentration. Moreover, defects in the OsST and OsMIK genes result in a great reduction of specific LPL components and LPL-phosphorus (LPL-P) contents in rice grain. In contrast, defective OsMRP5 and Os2-PGK genes led to a significant increase in individual LPL components. The effect of the Os2-PGK gene on the LPL accumulation was validated using breeding lines derived from a cross between KBNT-lpa (Os2-PGK mutation) and Jiahe218. This study demonstrates that these rice lpa mutants lead to the redistribution of Pi in endosperm and modify LPL biosynthesis. Increase LPLs in the endosperm in the lpa mutants may have practical applications in rice breeding to produce “healthier” rice.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.7b01576