Bioanalytical Method for the Determination of Hydroxyproline in Mouse Kidney by High-Performance Liquid Chromatography with Tandem Mass Spectrometric Detection

An analytical method was developed and validated for the measurement of hydroxyproline (Hyp) levels in mouse kidney by high-performance liquid chromatography with tandem mass spectrometric detection (LC/MS/MS) using an analytical column specially designed for the LC/MS/MS analysis for intact amino a...

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Bibliographic Details
Published in:Analytical Sciences 2017/06/10, Vol.33(6), pp.719-722
Main Authors: SHIOTA, Rika, MORITA, Hirotoshi, MATSUMOTO, Tomoko, MORIMOTO, Atsushi, HAYAKAWA, Jun, OKA, Masako, KAMIMORI, Hiroshi
Format: Article
Language:English
Subjects:
Amino acids
Analytical Chemistry
Animals
Chemistry
Chromatography
Chromatography, High Pressure Liquid
Deuterium
High performance liquid chromatography
Hydrochloric acid
Hydroxyproline
Hydroxyproline - analysis
Hydroxyproline - metabolism
Ions
kidney
Kidney - chemistry
Kidneys
Linearity
Liquid chromatography
mass spectrometry
Mice
Molecular Structure
mouse
Spectrometry
Tandem Mass Spectrometry
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Summary:An analytical method was developed and validated for the measurement of hydroxyproline (Hyp) levels in mouse kidney by high-performance liquid chromatography with tandem mass spectrometric detection (LC/MS/MS) using an analytical column specially designed for the LC/MS/MS analysis for intact amino acids. Tissue hydrolyzed with hydrochloric acid could be directly injected into the LC/MS/MS, as well as separated and detected using the deuterium labelled Hyp as an internal standard. The calibration curve showed good linearity from 5 to 500 nmol/mg of tissue; the precision and accuracy, including within- and between-run, were less than 6% and within 100 ± 6%, respectively.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.33.719