Chondroitin Sulfate-Based Biomineralizing Surface Hydrogels for Bone Tissue Engineering

Chondroitin sulfate (CS) is the major component of glycosaminoglycan in connective tissue. In this study, we fabricated methacrylated PEGDA/CS-based hydrogels with varying CS concentration (0, 1, 5, and 10%) and investigated them as biomineralizing three-dimensional scaffolds for charged ion binding...

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Bibliographic Details
Published in:ACS applied materials & interfaces 2017-07, Vol.9 (26), p.21639-21650
Main Authors: Kim, Hwan D, Lee, Eunjee A, An, Young-Hyeon, Kim, Seunghyun L, Lee, Seunghun S, Yu, Seung Jung, Jang, Hae Lin, Nam, Ki Tae, Im, Sung Gap, Hwang, Nathaniel S
Format: Article
Language:English
Subjects:
Bone and Bones
Cell Differentiation
Cells, Cultured
Chondroitin Sulfates - chemistry
Humans
Hydrogels
Mesenchymal Stem Cells
Osteogenesis
Tissue Engineering
Tissue Scaffolds
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Summary:Chondroitin sulfate (CS) is the major component of glycosaminoglycan in connective tissue. In this study, we fabricated methacrylated PEGDA/CS-based hydrogels with varying CS concentration (0, 1, 5, and 10%) and investigated them as biomineralizing three-dimensional scaffolds for charged ion binding and depositions. Due to its negative charge from the sulfate group, CS exhibited an osteogenically favorable microenvironment by binding charged ions such as calcium and phosphate. Particularly, ion binding and distribution within negatively charged hydrogel was dependent on CS concentration. Furthermore, CS dependent biomineralizing microenvironment induced osteogenic differentiation of human tonsil-derived mesenchymal stem cells in vitro. Finally, when we transplanted PEGDA/CS-based hydrogel into a critical sized cranial defect model for 8 weeks, 10% CS hydrogel induced effective bone formation with highest bone mineral density. This PEGDA/CS-based biomineralizing hydrogel platform can be utilized for in situ bone formation in addition to being an investigational tool for in vivo bone mineralization and resorption mechanisms.
ISSN:1944-8244
1944-8252
DOI:10.1021/acsami.7b04114