Extracellular redox cycling and hydroxyl radical production occurs widely in lichenized Ascomycetes

• Published in: Fungal biology 2017-06, Vol.121 (6-7), p.582-588
• Main Authors: Moyo, Calvin E., Beckett, Richard P., Trifonova, Tatyana V., Minibayeva, Farida V.
• Format: Article
• Language: English
• Subjects: Ascomycota - metabolism; Benzoquinones - metabolism; Ferric Compounds - metabolism; Hydroxyl Radical - metabolism; Laccases; Lichens - metabolism; Oxidation-Reduction; Peroxidases; Quinone reductases; Reactive oxygen species; Saprophyte
• ISSN: 1878-6146; 1878-6162
• DOI: 10.1016/j.funbio.2017.03.005

Some free-living Ascomycetes and white and brown rot Basidiomycetes can generate hydroxyl radicals using extracellular redox cycling. However, the mechanisms of hydroxyl radical production differ between white and brown rot Basidiomycetes, and are unknown for Ascomycetes. Here, we present a survey of extracellular hydroxyl radical production by a range of lichenized Ascomycetes. Results show that given a quinone and chelated ferric ions, many lichens can readily produce hydroxyl radicals, and this is accompanied by the reduction of Fe3+ to Fe2+. In white rot fungi, extracellular redox enzymes have been proposed to be involved in hydroxyl radical generation. However, a survey of a wide range of lichens suggests that in these fungi hydroxyl radical production does not directly correlate with the activity of laccases and peroxidases. Rather, radicals are probably produced by a mechanism like that proposed for brown rot fungi. Potential roles of hydroxyl radicals produced by lichens include the breakdown of lignocellulosic residues in the soil which may allow lichens to live a partially saprotrophic existence, the breakdown of toxic soil chemicals and the formation of an ‘oxidative burst’ to deter potential pathogens.