Molecular occurrence of trypanosomes, erythrocyte and serum sialic acid concentrations of Muturu and Bunaji cattle in Benue State, Nigeria

• Published in: Veterinary parasitology 2017-08, Vol.242, p.10-13
• Main Authors: Ode, Samuel, Adamu, Mathew, Taioe, Moeti, Thekisoe, Oriel, Adamu, Sani, Saror, Daniel I.
• Format: Article
• Language: English
• Subjects: Animals; Bunaji; Cattle; Cattle Diseases - blood; Cattle Diseases - epidemiology; Cattle Diseases - genetics; Cattle Diseases - parasitology; Erythrocyte sialic acid; Erythrocytes - physiology; Genetic Predisposition to Disease; Muturu; N-Acetylneuraminic Acid - blood; Nigeria - epidemiology; Phylogeny; Polymerase chain reaction; Serum sialic acid; Trypanosoma; Trypanosoma - isolation & purification; Trypanosomiasis - blood; Trypanosomiasis - epidemiology; Trypanosomiasis - parasitology; Trypanosomiasis - veterinary
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2017.05.018

•The Trypanosoma species circulating in Benue State, as detected in the two breeds of cattle, were Trypanosoma congolense, T. vivax, T. brucei and T. evansi.•This study, reports for the first time a natural infection of cattle with T. evansi and the use of a novel PCR in the diagnosis of trypanosome infections in cattle in Benue State, Nigeria.•The determination of the ESSA and FSSA concentrations in Muturu cattle in Nigeria is also reported for the first time. The Muturu cattle have a significantly higher ESSA than the Bunaji cattle, this may be responsible for their relative trypanotolerance.•This a novel report of FSSA concentrations of Muturu and Bunaji cattle, which may serve as baseline data that may be used in the diagnosis of diseases that cause alterations in this parameter in these breeds. One hundred each, of Muturu and Bunaji cattle were screened, using polymerase chain reaction (PCR), for trypanosomes in Makurdi and Gboko Local Government Areas of Benue State, Nigeria. Erythrocyte surface sialic acid (ESSA) and free serum sialic acid (FSSA) concentrations were determined and compared in both breeds with the aim of providing baseline data for research and diagnostic purposes. Five per cent (5%) and 23% of the Muturu and Bunaji cattle, respectively, were positive for trypanosomes. The result at p=0.005 was significantly different, with p value of 0.0002 and odd ratio of 0.1762. The Trypanosoma species circulating in Benue State, as detected in the two breeds of cattle, were Trypanosoma congolense, T. vivax, T. brucei and T. evansi. This study, therefore, reports for the first time a natural infection of cattle with T. evansi and the use of a novel PCR in the diagnosis of trypanosome infections in cattle in Benue State, Nigeria. The determination of the ESSA and FSSA concentrations in Muturu cattle in Nigeria is also reported for the first time. The Muturu cattle have a significantly higher ESSA than the Bunaji cattle, this may be responsible for their relative trypanotolerance.