The Inclusion of Chitosan in Poly-ε-caprolactone Nanoparticles: Impact on the Delivery System Characteristics and on the Adsorbed Ovalbumin Secondary Structure

• Published in: AAPS PharmSciTech 2018, Vol.19 (1), p.101-113
• Main Authors: Jesus, Sandra, Fragal, Elizangela H., Rubira, Adley F., Muniz, Edvani C., Valente, Artur J. M., Borges, Olga
• Format: Article
• Language: English
• Subjects: Adsorption; Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Biotechnology; Caproates - chemistry; Chitosan - chemistry; Drug Carriers - chemistry; Female; Hydrophobic and Hydrophilic Interactions; Lactones - chemistry; Mice; Mice, Inbred C57BL; Nanoparticles - chemistry; Ovalbumin - chemistry; Pharmacology/Toxicology; Pharmacy; Polymers - chemistry; Protein Structure, Secondary; Research Article
• ISSN: 1530-9932; 1530-9932
• DOI: 10.1208/s12249-017-0822-1

This report extensively explores the benefits of including chitosan into poly-ε-caprolactone (PCL) nanoparticles (NPs) to obtain an improved protein/antigen delivery system. Blend NPs (PCL/chitosan NPs) showed improved protein adsorption efficacy (84%) in low shear stress and aqueous environment, suggesting that a synergistic effect between PCL hydrophobic nature and the positive charges of chitosan present at the particle surface was responsible for protein interaction. Additionally, thermal analysis suggested the blend NPs were more stable than the isolated polymers and cytotoxicity assays in a primary cell culture revealed chitosan inclusion in PCL NPs reduced the toxicity of the delivery system. A quantitative 6-month stability study showed that the inclusion of chitosan in PCL NPs did not induce a change in adsorbed ovalbumin (OVA) secondary structure characterized by the increase in the unordered conformation (random coil), as it was observed for OVA adsorbed to chitosan NPs. Additionally, the slight conformational changes occurred, are not expected to compromise ovalbumin secondary structure and activity, during a 6-month storage even at high temperatures (45°C). In simulated biological fluids, PCL/chitosan NPs showed an advantageous release profile for oral delivery. Overall, the combination of PCL and chitosan characteristics provide PCL/chitosan NPs valuable features particularly important to the development of vaccines for developing countries, where it is difficult to ensure cold chain transportation and non-parenteral formulations would be preferred.