Three-phase partitioning as an elegant and versatile platform applied to nonchromatographic bioseparation processes

In recent years, a big trend has been the development of rapid, green, efficient, economical, and scalable approaches for the separation and purification of bioactive molecules from natural sources, which can be used in food, cosmetics, and medicine. As a new nonchromatographic bioseparation technol...

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Bibliographic Details
Published in:Critical reviews in food science and nutrition 2018-09, Vol.58 (14), p.2416-2431
Main Authors: Yan, Jing-Kun, Wang, Yao-Yao, Qiu, Wen-Yi, Ma, Haile, Wang, Zhen-Bin, Wu, Jian-Yong
Format: Article
Language:English
Subjects:
Ammonium
Ammonium sulfate
Biological activity
bioseparation
Butanol
Chemical partition
Cosmetics
enzyme
Food
Organic compounds
Partitioning
Polysaccharides
protein
Protein purification
Proteins
Purification
Reviews
Scientists
Separation
Sustainable development
Technology
Tert-butanol
Three-phase partitioning
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Summary:In recent years, a big trend has been the development of rapid, green, efficient, economical, and scalable approaches for the separation and purification of bioactive molecules from natural sources, which can be used in food, cosmetics, and medicine. As a new nonchromatographic bioseparation technology, three-phase partitioning (TPP) is attracting the attention of a growing number of scientists and engineers. Although a number of studies have been published in the last 40 years regarding the extraction, separation, and purification of numerous bioactive molecules using TPP systems, a background review on TPP partitioning fundamentals and its applications is much needed. Therefore, the present review focuses in detail on the TPP separation process, including the definition of TPP, partitioning mechanisms, parameters for establishing the suitable condition to form precipitate such as concentration of ammonium sulfate, content of tert-butanol, pH and temperature, and the application for separation and purification of protein, enzyme, plant oil, polysaccharide, and other small molecule organic compounds. In addition, the possible directions of future developments in TPP technology are discussed. The review presents a good opportunity, as well as a challenge for scientists, to understand the detailed partitioning rule and to take better use of TPP for the production and separation of various bioactive molecules, which have been intensively applied in the food and medical fields.
ISSN:1040-8398
1549-7852
DOI:10.1080/10408398.2017.1327418