Putty-like bone fillers based on CaP ceramics or Biosilicate® combined with carboxymethylcellulose: Characterization, optimization, and evaluation

Calcium phosphates and bioactive glass ceramics have been considered promising biomaterials for use in surgeries. However, their moldability should be further enhanced. We here thereby report the handling, physicochemical features, and morphological characteristics of formulations consisting of carb...

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Bibliographic Details
Published in:Journal of biomaterials applications 2017-08, Vol.32 (2), p.276-288
Main Authors: Gabbai-Armelin, Paulo R, Renno, Ana CM, Crovace, Murilo C, Magri, Angela MP, Zanotto, Edgar D, Peitl, Oscar, Leeuwenburgh, Sander CG, Jansen, John A, van den Beucken, Jeroen JJP
Format: Article
Language:English
Subjects:
Animals
Bone Substitutes - chemistry
Calcium Phosphates - chemistry
Carboxymethylcellulose Sodium - analogs & derivatives
Cell Line
Cell Proliferation
Cell Survival
Durapatite - chemistry
Elasticity
Glass - chemistry
Glycerol - chemistry
Mice
Viscosity
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Summary:Calcium phosphates and bioactive glass ceramics have been considered promising biomaterials for use in surgeries. However, their moldability should be further enhanced. We here thereby report the handling, physicochemical features, and morphological characteristics of formulations consisting of carboxymethylcellulose–glycerol and hydroxyapatite-tricalcium phosphate or Biosilicate® particles. We hypothesized that combining either material with carboxymethylcellulose–glycerol would improve handling properties, retaining their bioactivity. In addition to scanning electron microscopy, cohesion, mineralization, pH, and viscoelastic properties of the novel formulations, cell culture experiments were performed to evaluate the cytotoxicity and cell proliferation. Putty-like formulations were obtained with improved cohesion and moldability. Remarkably, mineralization in simulated body fluid of hydroxyapatite-tricalcium phosphate/carboxymethylcellulose–glycerol formulations was enhanced compared to pure hydroxyapatite-tricalcium phosphate. Cell experiments showed that all formulations were noncytotoxic and that HA-TCP60 and BGC50 extracts led to an increased cell proliferation. We conclude that combining carboxymethylcellulose–glycerol with either hydroxyapatite-tricalcium phosphate or Biosilicate® allows for the generation of moldable putties, improves handling properties, and retains the ceramic bioactivity.
ISSN:0885-3282
1530-8022
DOI:10.1177/0885328217713354