Clinical relevance of P-glycoprotein activity on peripheral blood mononuclear cells and polymorphonuclear neutrophils to methotrexate in systemic lupus erythematosus patients

To elucidate the relationship between P-glycoprotein activity on peripheral blood leukocytes of systemic lupus erythematosus (SLE) patients with lupus arthritis and the clinical response to methotrexate. An observational study was made in patients with SLE according to ACR criteria 1997 who had arth...

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Bibliographic Details
Published in:Clinical rheumatology 2017-10, Vol.36 (10), p.2267-2272
Main Authors: García-Carrasco, Mario, Mendoza-Pinto, Claudia, Macías-Díaz, Salvador, Etchegaray-Morales, Ivet, Méndez-Martínez, Socorro, Soto-Santillán, Pamela, Pérez-Romano, Beatriz, Jiménez-Herrera, Erick A., Guzmán-Ruiz, Omar, Ruiz-Argüelles, Alejandro
Format: Article
Language:English
Subjects:
Adult
Arthralgia
Arthritis
ATP-Binding Cassette, Sub-Family B, Member 1 - blood
Female
Fluorescence
Glycoproteins
Humans
Leukocytes (mononuclear)
Leukocytes (neutrophilic)
Leukocytes (polymorphonuclear)
Leukocytes, Mononuclear - cytology
Lupus
Lupus Erythematosus, Systemic - blood
Lupus Erythematosus, Systemic - drug therapy
Male
Medicine
Medicine & Public Health
Methotrexate
Methotrexate - pharmacology
Middle Aged
Neutrophils
Neutrophils - cytology
Original Article
P-Glycoprotein
Peripheral blood mononuclear cells
Rheumatology
Severity of Illness Index
Systemic lupus erythematosus
Treatment Outcome
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Summary:To elucidate the relationship between P-glycoprotein activity on peripheral blood leukocytes of systemic lupus erythematosus (SLE) patients with lupus arthritis and the clinical response to methotrexate. An observational study was made in patients with SLE according to ACR criteria 1997 who had arthralgia and arthritis and received methotrexate for ≥3 months. Methotrexate responders and non-responders were compared according to the Clinical Disease Activity Index. Mononuclear cells and polymorphonuclear neutrophils were isolated from SLE patients and P-glycoprotein expression was measured using the relative fluorescence index and percentage of positive cells. The chi-square test was used to compare P-glycoprotein activity between responders and non-responders. Thirty-two patients with a mean age of 45.4 ± 10.7 years were included: 34.4% had a response to methotrexate and 65.6% did not. Mean relative fluorescence units of both mononuclear cells and polymorphonuclear neutrophils were significantly lower in patients with a good response (7.0 ± 4.3 vs. 9.6 ± 3.8; p  = 0.041 and 4.2 ± 3.5 vs. 7.6 ± 4.0; p  = 0.004). The prevalence of low fluorescence levels (
ISSN:0770-3198
1434-9949
DOI:10.1007/s10067-017-3728-0