Thiol-ene micropillar array electrospray ionization platform for zeptomole level bioanalysis

A micropillar array electrospray ionization (μPESI) platform fabricated from thiol-enes with 56 individual polyethylene glycol coated μPESI chips for bioanalytical mass spectrometry is introduced. Bioanalysis capability is shown by measurement of a protein, a protein digest and a cell lysate sample....

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Bibliographic Details
Published in:Analyst (London) 2017-07, Vol.142 (14), p.2552-2557
Main Authors: Lipponen, K, Tähkä, S, Sikanen, T, Jokinen, V, Tatikonda, A, Franssila, S, Kostiainen, R, Kotiaho, T
Format: Article
Language:English
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Summary:A micropillar array electrospray ionization (μPESI) platform fabricated from thiol-enes with 56 individual polyethylene glycol coated μPESI chips for bioanalytical mass spectrometry is introduced. Bioanalysis capability is shown by measurement of a protein, a protein digest and a cell lysate sample. The thiol-ene polyethylene glycol (PEG) coated μPESI chip allows the use of a wide range of aqueous-organic solvent compositions and provides a detection limit at 60 zeptomole level (6 × 10 mol) for a peptide standard.
ISSN:0003-2654
1364-5528
DOI:10.1039/c7an00544j