Rapid detection of Salmonella with Recombinase Aided Amplification

Rapid Salmonella detection using Recombinase Aided Amplification was established. The reaction completes in 20 min at 39°C and can be performed with a portable device. Once further improved, this method should be a great choice for monitoring contamination, such as foodborne Salmonella or for simila...

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Bibliographic Details
Published in:Journal of microbiological methods 2017-08, Vol.139, p.202-204
Main Authors: Zhang, Xiaoping, Guo, Lichuan, Ma, Ranran, Cong, Lijuan, Wu, Zhonghua, Wei, Ying, Xue, Shijie, Zheng, Wei, Tang, Saijun
Format: Article
Language:English
Subjects:
DNA Primers
Food Microbiology
Food safety
Foodborne
Limit of Detection
Nucleic Acid Amplification Techniques - instrumentation
Nucleic Acid Amplification Techniques - methods
Recombinase Aided Amplification
Recombinases - genetics
Salmonella
Salmonella - genetics
Salmonella - isolation & purification
Time Factors
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Summary:Rapid Salmonella detection using Recombinase Aided Amplification was established. The reaction completes in 20 min at 39°C and can be performed with a portable device. Once further improved, this method should be a great choice for monitoring contamination, such as foodborne Salmonella or for similar purposes. •Rapid detection of Salmonella by Recombinase Aided Amplification (RAA) was established.•Basic and real-time fluorescence detection reactions of Salmonella were reported.•These methods are sensitive, specific and more rapid.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2017.06.011