Transcriptomic response of the insect vector, Peregrinus maidis, to Maize mosaic rhabdovirus and identification of conserved responses to propagative viruses in hopper vectors

Abstract Maize mosaic virus (MMV) is a plant-pathogenic rhabdovirus that is transmitted by the corn planthopper, Peregrinus maidis , in a propagative manner. P. maidis supports long-term MMV infections with no negative effects on insect performance. To elucidate whole-body transcriptome responses to...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2017-09, Vol.509, p.71-81
Main Authors: Martin, Kathleen M, Barandoc-Alviar, Karen, Schneweis, Derek J, Stewart, Catherine L, Rotenberg, Dorith, Whitfield, Anna E
Format: Article
Language:English
Subjects:
Animals
Gene Expression Profiling
Hemiptera - genetics
Hemiptera - virology
Hemipteran
Infectious Disease
Insect Vectors - genetics
Insect Vectors - virology
Nucleorhabdovirus
Planthopper
Rhabdoviridae
Rhabdoviridae - immunology
Transcriptomics
Virus-vector interactions
Zea mays - virology
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Summary:Abstract Maize mosaic virus (MMV) is a plant-pathogenic rhabdovirus that is transmitted by the corn planthopper, Peregrinus maidis , in a propagative manner. P. maidis supports long-term MMV infections with no negative effects on insect performance. To elucidate whole-body transcriptome responses to virus infection, RNA-Seq was used to examine differential gene expression of virus-infected adult insects, and libraries were prepared from replicated groups of virus-exposed insects and non-exposed insects. From the 68,003 de novo -assembled transcripts, 144 were differentially-expressed (DE) during viral infection with comparable numbers up- and down-regulated. DE transcripts with similarity to genes associated with transposable elements (i.e., RNA-directed DNA polymerases) were enriched and may represent a mechanisim for modulating virus infection. Comparison of the P. maidis DE transcripts to published propagative virus-responsive transcript databases for two other hopper vectors revealed that 16% of the DE transcripts were shared across the three systems and may represent conserved responses to propagative viruses.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2017.05.019