Black-pigmented anaerobic bacteria associated with ovine periodontitis

• Published in: Veterinary microbiology 2017-05, Vol.203, p.271-274
• Main Authors: Borsanelli, Ana C., Gaetti-Jardim, Elerson, Schweitzer, Christiane M., Viora, Lorenzo, Busin, Valentina, Riggio, Marcello P., Dutra, Iveraldo S.
• Format: Article
• Language: English
• Subjects: Anaerobic bacteria; Animals; Bacteria; Bacteria, Anaerobic - genetics; Bacteria, Anaerobic - isolation & purification; Biofilms - growth & development; Culling; Gum disease; Infectious diseases; Lesions; Microorganisms; Occlusion; Ovis aries; Pathogenesis; Periodontal disease; Periodontal Pocket - microbiology; Periodontal Pocket - veterinary; Periodontitis; Periodontitis - microbiology; Periodontitis - veterinary; Pocket; Polymerase chain reaction; Polymerase Chain Reaction - veterinary; Porphyromonas; Porphyromonas - genetics; Porphyromonas - isolation & purification; Prevotella; Prevotella - genetics; Prevotella - isolation & purification; Primers; Risk analysis; Rumination; Sheep; Sheep Diseases - microbiology; Studies; Teeth
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2017.03.032

•Sheep periodontitis is considered one of the major reason for premature culling of animals in flocks and its etiology is not completely elucidated.•Species of Prevotella and Porphyromonas genera are important pathogens in human oral microbiome and this study identified the presence of some species of both genera in the subgingival biofilm of sheep.•Polymerase chain reaction enabled to verify the presence of different species of Prevotella and Porphyromonas genera in the periodontal pocket of sheep.•The occurrence of Poprhyromonas asaccharolytica, Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella buccae, Prevotella intermedia, Prevotella melaninogenica and Prevotella nigrescens is strongly associated with the lesions of sheep with periodontitis.•An analysis of detection frequency of different microorganisms using the Spearmańs correlation test showed association between members of the subgingival biofilm of sheep. Periodontitis is a polymicrobial infectious disease that causes occlusion change, tooth loss, difficulty in rumination, and premature culling of animals. This study aimed to detect species of the genera Porphyromonas and Prevotella present in the periodontal pocket of sheep with lesions deeper than 5mm (n=14) and in the gingival sulcus of animals considered periodontally healthy (n=20). The presence of microorganisms was evaluated by polymerase chain reaction (PCR) using specific primers for Porphyromonas asaccharolytica, Porphyromonas endodontalis, Porphyromonas gingivalis, Porphyromonas gulae, Prevotella buccae, Prevotella intermedia, Prevotella loescheii, Prevotella melaninogenica, Prevotella nigrescens, Prevotella oralis, and Prevotella tannerae. Prevalence and risk analysis were performed using Student’s t-test and Spearman’s correlation. Among the Prevotella and Porphyromonas species detected in the periodontal lesions of sheep, P. melaninogenica (85.7%), P. buccae (64.3%), P. gingivalis (50%), and P. endodontalis (50%) were most prevalent. P. gingivalis (15%) and P. oralis (10%) prevailed in the gingival sulcus. P. gulae and P. tannerae were not detected in the 34 samples studied. Data evaluation by t-test verified that occurrence of P. asaccharolytica, P. endodontalis, P. gingivalis, P. buccae, P. intermedia, P. melalinogenica, and P. nigrescens correlated with sheep periodontitis. The findings of this study will be an important contribution to research on pathogenesis of sheep periodontitis and development of its control m