Sciatic nerve regeneration by transplantation of in vitro differentiated nucleus pulposus progenitor cells

To assess the applicability of mouse intervertebral disc-derived nucleus pulposus (NP) progenitor cells as a cell source for sciatic nerve regeneration. P0-Cre/Floxed-EGFP-transgenic mouse-derived NP progenitor cells were differentiated to Schwann-like cells in conventional induction medium. Schwann...

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Bibliographic Details
Published in:Regenerative medicine 2017-04, Vol.12 (4), p.365-376
Main Authors: Ishii, Takayuki, Sakai, Daisuke, Schol, Jordy, Nakai, Tomoko, Suyama, Kaori, Watanabe, Masahiko
Format: Article
Language:English
Subjects:
Animals
Cell Differentiation
Colony-Forming Units Assay
Female
Immunohistochemistry
Mice, Transgenic
Nerve Regeneration
Nucleus Pulposus - transplantation
Recovery of Function
Schwann Cells - cytology
Schwann Cells - transplantation
Sciatic Nerve - physiopathology
Sciatic Nerve - ultrastructure
Stem Cell Transplantation
Stem Cells - cytology
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Summary:To assess the applicability of mouse intervertebral disc-derived nucleus pulposus (NP) progenitor cells as a cell source for sciatic nerve regeneration. P0-Cre/Floxed-EGFP-transgenic mouse-derived NP progenitor cells were differentiated to Schwann-like cells in conventional induction medium. Schwann-like cells were subsequently transplanted into a mouse model of sciatic nerve transection, and nerve regeneration assessed by immunohistochemistry, electron microscopy and functional walking track analysis and heat stimulus reflex. NP progenitor cells differentiated into Schwann-like cells. Transplantation of these cells promoted myelinated axon formation, morphology restoration and nerve function improvement. NP progenitor cells have the capacity to differentiate into neuronal cells and are candidates for peripheral nerve regeneration therapy.
ISSN:1746-0751
1746-076X
DOI:10.2217/rme-2016-0168