Evidence for altered levels of IgD in the nasal airway mucosa of patients with chronic rhinosinusitis

• Published in: Journal of allergy and clinical immunology 2017-12, Vol.140 (6), p.1562-1571.e5
• Main Authors: Min, Jin-Young, Nayak, Jayakar V., Hulse, Kathryn E., Stevens, Whitney W., Raju, Paul A., Huang, Julia H., Suh, Lydia A., Van Roey, Griet A., Norton, James E., Carter, Roderick G., Price, Caroline P.E., Weibman, Ava R., Rashan, Ali R., Ghosn, Eliver E., Patel, Zara M., Homma, Tetsuya, Conley, David B., Welch, Kevin C., Shintani-Smith, Stephanie, Peters, Anju T., Grammer, Leslie C., Harris, Kathleen E., Kato, Atsushi, Hwang, Peter H., Kern, Robert C., Herzenberg, Leonore A., Schleimer, Robert P., Tan, Bruce K.
• Format: Article
• Language: English
• Subjects: ADP-ribosyl Cyclase 1 - metabolism; Adult; Aged; Antibiotics; Antigens, CD19 - metabolism; B cells; B-Lymphocytes - immunology; Bacteria; bacterial infection; CD19 antigen; Cells, Cultured; Chronic Disease; Chronic rhinosinusitis; Cytokines; Enzyme-linked immunosorbent assay; Family medical history; Female; Flow cytometry; Humans; IgD; IL-2; Immune system; Immunoassays; Immunofluorescence; Immunoglobulin D; Immunoglobulin D - metabolism; Immunoglobulin M; Immunoglobulins; Inflammation; Interleukin 2; Interleukin-2 - metabolism; Lymphocytes B; Male; Measurement methods; Medical records; Medicine; Middle Aged; Moraxella catarrhalis; Mucosal immunity; Nasal Mucosa - immunology; Nasal Polyps - immunology; Otolaryngology; Pathogenesis; Polyps; Proteins; Respiratory System - pathology; Respiratory tract; Rhinitis; Rhinitis - immunology; Rhinosinusitis; Sinuses; Sinusitis; Sinusitis - immunology; Studies; Tissues; Up-Regulation; Young Adult
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2017.05.032

IgD is an enigmatic antibody isotype best known when coexpressed with IgM on naive B cells. However, increased soluble IgD (sIgD) levels and increased IgD+IgM− B-cell populations have been described in the human upper respiratory mucosa. We assessed whether levels of sIgD and IgD+ B cell counts are altered in nasal tissue from patients with chronic rhinosinusitis (CRS). We further characterized IgD+ B-cell populations and explored clinical and local inflammatory factors associated with tissue sIgD levels. sIgD levels were measured by means of ELISA in nasal tissues, nasal lavage fluid, sera, and supernatants of dissociated nasal tissues. IgD+ cells were identified by using immunofluorescence and flow cytometry. Inflammatory mediator levels in tissues were assessed by using real-time PCR and multiplex immunoassays. Bacterial cultures from the middle meatus were performed. Underlying medical history and medicine use were obtained from medical records. sIgD levels and numbers of IgD+ cells were significantly increased in uncinate tissue (UT) of patients with chronic rhinosinusitis without nasal polyps (CRSsNP) compared with that of control subjects (4-fold, P