TCR+ CD3+ CD4− CD8− effector T cells in psoriasis

Abstract The autoimmune/inflammatory disorder psoriasis is characterized by keratinocyte proliferation and immune cell infiltration of the skin. TCR+ CD3+ CD4− CD8− “double negative” (DN) T cells can derive from CD8+ T cells through the down-regulation of CD8. The inhibitory molecule programmed deat...

Full description

Saved in:
Bibliographic Details
Published in:Clinical immunology (Orlando, Fla.) Fla.), 2017-08, Vol.181, p.51-59
Main Authors: Brandt, D, Sergon, M, Abraham, S, Mäbert, K, Hedrich, C.M
Format: Article
Language:English
Subjects:
Allergy and Immunology
Case-Control Studies
CD3 Complex - metabolism
CD4 Antigens - metabolism
CD8 Antigens - metabolism
DNA Methylation
Double negative T cells
Effector memory T cells
Enzyme-Linked Immunosorbent Assay
Epigenesis, Genetic
Flow Cytometry
Humans
IFN-γ
Immunohistochemistry
Interferon-gamma - immunology
Leukocyte Common Antigens - metabolism
PD-1
Programmed Cell Death 1 Receptor - immunology
Programmed death-1
Psoriasis
Psoriasis - genetics
Psoriasis - immunology
Real-Time Polymerase Chain Reaction
Receptors, Antigen, T-Cell - metabolism
Receptors, CCR7 - metabolism
Skin - immunology
Skin - metabolism
T-Lymphocyte Subsets - immunology
T-Lymphocyte Subsets - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract The autoimmune/inflammatory disorder psoriasis is characterized by keratinocyte proliferation and immune cell infiltration of the skin. TCR+ CD3+ CD4− CD8− “double negative” (DN) T cells can derive from CD8+ T cells through the down-regulation of CD8. The inhibitory molecule programmed death (PD-)1 is expressed on activated T cells and plays a role in the maintenance of peripheral tolerance. A subset of DN T cells, characterized by the expression of PD-1, has recently been demonstrated to be self-reactive. We demonstrate that a majority of DN T cells exhibits effector memory phenotypes, express IFN-γ, and fail to proliferate. DN T cells from psoriasis patients are characterized by reduced DNA methylation of the IFNG gene and increased PD-1 expression. Furthermore, PD-1 positive DN T cells infiltrate the epidermis in psoriatic skin lesions. Our observations offer additional insight into the molecular pathophysiology of plaque psoriasis and show promise as potential disease biomarkers and/or therapeutic targets for future interventions.
ISSN:1521-6616
1521-7035
DOI:10.1016/j.clim.2017.06.002