Expression profiles of genes for mitochondrial respiratory energy-dissipating systems and antioxidant enzymes in wheat leaves during de-etiolation

• Published in: Journal of plant physiology 2017-08, Vol.215, p.110-121
• Main Authors: Garmash, Elena V., Velegzhaninov, Ilya O., Grabelnych, Olga I., Borovik, Olga A., Silina, Ekaterina V., Voinikov, Victor K., Golovko, Tamara K.
• Format: Article
• Language: English
• Subjects: Alternative oxidase; Antioxidants; Antioxidants - metabolism; AOX; Coding; Darkness; De-etiolation; Dissipation; Energy; Energy-dissipating respiratory components; Enzymes; Etiolation; Etiolation - genetics; Etiolation - physiology; Gene expression; Gene Expression Regulation, Plant - genetics; Gene Expression Regulation, Plant - physiology; Genes; Mitochondria; Mitochondria - metabolism; Mitochondrial Proteins - genetics; Mitochondrial Proteins - metabolism; Oxidative stress; Oxidative Stress - genetics; Oxidative Stress - physiology; Oxidoreductases - genetics; Oxidoreductases - metabolism; Photosynthesis; Photosynthesis - genetics; Photosynthesis - physiology; Photosynthetic apparatus; Plant Leaves - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Reactive oxygen species; Reactive Oxygen Species - metabolism; Respiration; Seedlings; Spring wheat; Triticum - metabolism; Triticum aestivum; Wheat
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2017.05.023

Mitochondrial respiratory components participate in the maintenance of chloroplast functional activity. This study investigates the effects 48h de-etiolation of spring wheat seedlings (Triticum aestivum L., var. Irgina) on the expression of genes that encode energy-dissipating respiratory components and antioxidant enzymes under continuous light conditions. The expression of AOX1a following the prolonged darkness exhibited a pattern indicating a prominent dependence on light. The expression of other respiratory genes, including NDA2, NDB2, and UCP1b, increased during de-etiolation and dark-to-light transition; however, changes in the expression of these genes occurred later than those in AOX1a expression. A high expression of NDA1 was detected after 12h of de-etiolation. The suppression of AOX1a, NDA2, NDB2, and UCP1b was observed 24h after de-etiolation when the photosynthetic apparatus and its defence systems against excess light were completely developed. The expression patterns of the respiratory genes and several genes encoding antioxidant enzymes (MnSOD, Cu-ZnSOD, t-APX, GR, and GRX) were quite similar. Our data indicate that the induction of nuclear genes encoding respiratory and antioxidant enzymes allow the plants to control reactive oxygen species (ROS) production and avoid oxidative stress during de-etiolation.