Alginate oligosaccharides modify hyphal infiltration of Candida albicans in an in vitro model of invasive human candidosis

Aims A novel alginate oligomer (OligoG CF‐5/20) has been shown to potentiate antifungal therapy against a range of fungal pathogens. The current study assessed the effect of this oligomer on in vitro virulence factor expression and epithelial invasion by Candida species. Methods and Results Plate su...

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Bibliographic Details
Published in:Journal of applied microbiology 2017-09, Vol.123 (3), p.625-636
Main Authors: Pritchard, M.F., Jack, A.A., Powell, L.C., Sadh, H., Rye, P.D., Hill, K.E., Thomas, D.W.
Format: Article
Language:English
Subjects:
Alginates - pharmacology
Antifungal Agents - pharmacology
antimicrobials
biofilms
Candida albicans - drug effects
Candida albicans - genetics
Candida albicans - growth & development
Candida albicans - metabolism
Candidiasis - drug therapy
Candidiasis - microbiology
fungi
Glucuronic Acid - pharmacology
Hexuronic Acids - pharmacology
Humans
Hyphae - drug effects
Hyphae - growth & development
infection
Oligosaccharides - pharmacology
virulence
Virulence Factors - genetics
Virulence Factors - metabolism
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Summary:Aims A novel alginate oligomer (OligoG CF‐5/20) has been shown to potentiate antifungal therapy against a range of fungal pathogens. The current study assessed the effect of this oligomer on in vitro virulence factor expression and epithelial invasion by Candida species. Methods and Results Plate substrate assays and epithelial models were used to assess Candida albicans (CCUG 39343 and ATCC 90028) invasion, in conjunction with confocal laser scanning microscopy and histochemistry. Expression of candidal virulence factors was determined biochemically and by quantitative PCR (qPCR). Changes in surface charge of C. albicans following OligoG treatment were analysed using electrophoretic light scattering. OligoG induced marked alterations in hyphal formation in the substrate assays and reduced invasion in the epithelial model (P 
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.13516