The effect of antimicrobial photodynamic therapy on the expression of novel methicillin resistance markers determined using cDNA-AFLP approach in Staphylococcus aureus

Highlights • cDNA-AFLP is suitable approach for identification of new target genes implicated in antimicrobial resistance. • Serine hydrolase family protein (Shfp) encoding gene was up-regulated in methicillin resistant Staphylococcus aureus (MRSA). • The Shfp encoding genes might be implicated in m...

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Bibliographic Details
Published in:Photodiagnosis and photodynamic therapy 2017-09, Vol.19, p.249-255
Main Authors: Hoorijani, Mohammad Neshvan, Rostami, Hosein, Pourhajibagher, Maryam, Chiniforush, Nasim, Heidari, Mansour, Pourakbari, Babak, Kazemian, Hossein, Davari, Kambiz, Amini, Vahid, Raoofian, Reza, Bahador, Abbas
Format: Article
Language:English
Subjects:
Amplified Fragment Length Polymorphism Analysis
Antimicrobial photodynamic therapy
CDNA-AFLP
Diode laser
DNA, Complementary
Hematology, Oncology and Palliative Medicine
Humans
Internal Medicine
Methicillin Resistance - drug effects
Methicillin Resistance - genetics
Methicillin-Resistant Staphylococcus aureus - drug effects
Methicillin-Resistant Staphylococcus aureus - genetics
Microbial Sensitivity Tests
MRSA
Photochemotherapy - methods
Photosensitizing Agents - pharmacology
Polymorphism, Single Nucleotide
qRT-PCR
Staphylococcus aureus
Tolonium Chloride - pharmacology
Toluidine blue O
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Summary:Highlights • cDNA-AFLP is suitable approach for identification of new target genes implicated in antimicrobial resistance. • Serine hydrolase family protein (Shfp) encoding gene was up-regulated in methicillin resistant Staphylococcus aureus (MRSA). • The Shfp encoding genes might be implicated in methicillin resistance in MRSA. • A conserved hypothetical protein (Chp) encoding gene that was up-regulated in MRSA might be implicated in methicillin resistance in MRSA. • sPDT would lead to reduction in resistance to methicillin in MRSA by suppressing the expression of the Shfp and Chp encoding genes.
ISSN:1572-1000
1873-1597
DOI:10.1016/j.pdpdt.2017.06.012