Three-Dimensional Localization of an Individual Fluorescent Molecule with Angstrom Precision

Among imaging techniques, fluorescence microscopy is a unique method to noninvasively image individual molecules in whole cells. If the three-dimensional spatial precision is improved to the angstrom level, various molecular arrangements in the cell can be visualized on an individual basis. We have...

Full description

Saved in:
Bibliographic Details
Published in:Journal of the American Chemical Society 2017-07, Vol.139 (26), p.8990-8994
Main Authors: Furubayashi, Taku, Motohashi, Kazuya, Wakao, Keisuke, Matsuda, Tsuyoshi, Kii, Isao, Hosoya, Takamitsu, Hayashi, Nobuhiro, Sadaie, Mahito, Ishikawa, Fuyuki, Matsushita, Michio, Fujiyoshi, Satoru
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Among imaging techniques, fluorescence microscopy is a unique method to noninvasively image individual molecules in whole cells. If the three-dimensional spatial precision is improved to the angstrom level, various molecular arrangements in the cell can be visualized on an individual basis. We have developed a cryogenic reflecting microscope with a numerical aperture of 0.99 and an imaging stability of 0.05 nm in standard deviation at a temperature of 1.8 K. The key optics to realize the cryogenic performances is the reflecting objective developed by our laboratory. With this cryogenic microscope, an individual fluorescent molecule (ATTO647N) at 1.8 K was localized with standard errors of 0.53 nm (x), 0.31 nm (y), and 0.90 nm (z) when 106 fluorescence photons from the molecule were accumulated in 5 min.
ISSN:0002-7863
1520-5126
DOI:10.1021/jacs.7b03899