Phenotypic Analysis of Organoids by Proteomics

The development of 3D cell cultures into self‐organizing organ‐like structures named organoids provides a model that better reflects in vivo organ physiology and their functional properties. Organoids have been established from several organs, such as the intestine, prostate, brain, liver, kidney an...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2017-10, Vol.17 (20), p.n/a
Main Authors: Gonneaud, Alexis, Asselin, Claude, Boudreau, François, Boisvert, François‐Michel
Format: Article
Language:English
Subjects:
3D cell culture
Animals
Brain
Brain - cytology
Brain - growth & development
Cell culture
Chromatin
Deoxyribonucleic acid
Digestive System - cytology
Digestive System - growth & development
DNA
Humans
Intestine
Kidney - cytology
Kidney - growth & development
Kidneys
Liver
Mass Spectrometry
Mass spectroscopy
Organ Culture Techniques - methods
Organogenesis
Organoids
Organoids - cytology
Organoids - growth & development
Organs
Pancreas
Peptides - analysis
Phenotype
Prostate
Proteome - analysis
Proteomics
Proteomics - methods
Quantitative proteomics
SILAC
Stem cells
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Summary:The development of 3D cell cultures into self‐organizing organ‐like structures named organoids provides a model that better reflects in vivo organ physiology and their functional properties. Organoids have been established from several organs, such as the intestine, prostate, brain, liver, kidney and pancreas. With recent advances in high‐throughput and ‐omics profiling technologies, it is now possible to study the mechanisms of cellular organisation at the systems level. It is therefore not surprising that these methods are now used to characterize organoids at the transcriptomic, proteomic, chromatin state and transcription factor DNA‐binding levels. These approaches can therefore provide a wealth of information regarding both the mechanisms involved in different diseases, and those involved in cell responses to different conditions, in a more in vivo setting. The authors provide an overview of the potential applications of quantitative mass spectrometry with organoid culture, and how the use of large‐scale proteome measurements is emerging in different organoid systems.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.201700023