Isolation of anticancer and antimicrobial metabolites from Epicoccum nigrum; endophyte of Ferula sumbul

Owing to the importance of endophytes, current research was aimed to purify the secondary metabolites from targeted source. Ferula sumbul, a lipophilic extract of the endophyte was prepared in 10% methanol and partitioned with ethyl acetate and bioassay guided isolation was carried using standard pr...

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Bibliographic Details
Published in:Microbial pathogenesis 2017-09, Vol.110, p.214-224
Main Authors: Perveen, Irum, Raza, Muhammad Asam, Iqbal, Tahir, Naz, Iffat, Sehar, Shama, Ahmed, Safia
Format: Article
Language:English
Subjects:
Anti-Infective Agents - chemistry
Anti-Infective Agents - isolation & purification
Anti-Infective Agents - pharmacology
Anticancer
Antineoplastic Agents - chemistry
Antineoplastic Agents - isolation & purification
Antineoplastic Agents - pharmacology
Ascomycota - chemistry
Ascomycota - metabolism
Bacteria - drug effects
Cell Line, Tumor - drug effects
Cell Survival - drug effects
Endophytes - chemistry
Endophytes - classification
Endophytes - metabolism
Epicoccum nigrum
Ferula - microbiology
Ferula sumbul
Fungi - drug effects
Humans
Inhibitory Concentration 50
Melanoma
MTT
Phthalic Acids - chemistry
Phthalic Acids - pharmacology
Phylogeny
Plant Roots - microbiology
Plants derived drugs
Prodigiosin - analogs & derivatives
Prodigiosin - chemistry
Prodigiosin - pharmacology
Terpenes - chemistry
Terpenes - pharmacology
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Summary:Owing to the importance of endophytes, current research was aimed to purify the secondary metabolites from targeted source. Ferula sumbul, a lipophilic extract of the endophyte was prepared in 10% methanol and partitioned with ethyl acetate and bioassay guided isolation was carried using standard protocols against bacterial, fungal and cancer cells. The active fractions consisted of three new metabolites (2-methyl-3-nonyl prodiginine, Bis (2-ethylhexyl) phthalate, and a meroterpenoid, Preaustinoid A). Their structures were confirmed with LCMS/MS. The purified metabolites showed valuable results against tested activities which concluded that these compounds have great potential and these may be applicable to textile (dyeing), pharmaceutical (drug, infectious agents) and food (preservatives) industries. This study reveals the potential of E. nigrum as an important source of bioactive compounds including 2-methyl-3-nonyl prodiginine, Bis (2-ethylhexyl) phthalate, and Preaustinoid A. This is first report of isolation of prodiginines as well as meroterpenoid and Bis (2-ethylhexyl) phthalate from Epicoccum nigrum.
ISSN:0882-4010
1096-1208
DOI:10.1016/j.micpath.2017.06.033