Inferior olivary projection to the zebrin II stripes in lobule IXcd of the pigeon flocculus: A retrograde tracing study

• Published in: Journal of comparative neurology (1911) 2017-10, Vol.525 (14), p.3158-3173
• Main Authors: Wylie, Douglas R., Gutiérrez‐Ibáñez, Cristián, Corfield, Jeremy R., Craciun, Iulia, Graham, David J., Hurd, Peter L.
• Format: Article
• Language: English
• Subjects: Aldolase; aldolase C; Animals; Avian Proteins - metabolism; Cerebellar Vermis - anatomy & histology; Cerebellar Vermis - metabolism; cerebellum; climbing fiber; Columbidae - anatomy & histology; Columbidae - metabolism; Eye; Firing pattern; Immunohistochemistry; inferior olive; Nerve Tissue Proteins - metabolism; Neural Pathways - anatomy & histology; Neural Pathways - metabolism; Neuroanatomical Tract-Tracing Techniques; Olivary Nucleus - anatomy & histology; Olivary Nucleus - metabolism; optic flow; optokinetic; Purkinje cells; RRID:AB_2242641; RRID:AB_2340428; RRID:AB_2340432; Visual Perception - physiology; Zebrin II
• ISSN: 0021-9967; 1096-9861
• DOI: 10.1002/cne.24270

Zebrin II (ZII; a.k.a. aldolase C) is expressed heterogeneously in Purkinje cells (PCs) such that there are sagittal stripes of high expression (ZII+) interdigitated with stripes of little or no expression (ZII−). The pigeon flocculus receives visual‐optokinetic information and is important for generating compensatory eye movements. It consists of 4 sagittal zones based on PC complex spike activity (CSA) in response to rotational optokinetic stimuli. There are two zones where CSA responds best to rotation about the vertical axis (VA), interdigitated with two zones where CSA responds best to rotation about an horizontal axis (HA). These optokinetic zones relate to the ZII stripes in folium IXcd of the flocculus, such that an optokinetic zone spans a ZII+/− pair: the HA zones span the P5+/− and P7+/− ZII stripe pairs, whereas the VA zones correspond to ZII stripe pairs P4+/− and P6+/−. In the present study, we used fluorescent retrograde tracing to determine the olivary inputs to the ZII+ and ZII− stripes within the functional pairs. We found that separate but adjacent areas of the medial column of the inferior olive (mcIO) project to the ZII+ and ZII− stripes within each of the functional pairs. Thus, although a ZII+/− stripe pair represents a functional unit in the pigeon flocculus insofar as the CSA of all PCs in the stripe pair encodes similar sensory information, the olivary inputs to the ZII+ and ZII− stripes arise from different, although adjacent, regions of the mcIO. We show a topographical relationship between regions in the medial column of the inferior olive in pigeons to parasagittal stripes in the flocculus defined by zebrin II expression. There are stripes that show ZII immunopositive (+) alternating with those that are ZII immunonegative (–).