Genetic diversity of human metapneumovirus in hospitalized children with acute respiratory infections in Croatia

Human metapneumovirus (HMPV) is recognized as a global and frequent cause of acute respiratory tract infections among people of all ages. The objectives of this study were molecular epidemiology and evolutionary analysis of HMPV strains which produced moderate and severe acute respiratory tract infe...

Full description

Saved in:
Bibliographic Details
Published in:Journal of medical virology 2017-11, Vol.89 (11), p.1885-1893
Main Authors: Jagušić, Maja, Slović, Anamarija, Ljubin‐Sternak, Sunčanica, Mlinarić‐Galinović, Gordana, Forčić, Dubravko
Format: Article
Language:English
Subjects:
Acute Disease
Age
Bronchitis - epidemiology
Bronchitis - virology
Child
Child, Hospitalized - statistics & numerical data
Child, Preschool
Children
Circulation
Croatia - epidemiology
Epidemiology
Evolution
F gene
Female
fusion protein
Gene sequencing
Genetic diversity
genetic variability
Genetic Variation
Genomes
Genotype
glycoproteins
human metapneumovirus
Humans
Immunofluorescence
Infant
Infant, Newborn
Infections
Male
Metapneumovirus - genetics
Paramyxoviridae Infections - epidemiology
Paramyxoviridae Infections - virology
Phylogeny
Pneumonia, Viral - epidemiology
Pneumonia, Viral - virology
Prevalence
Respiratory tract
Respiratory tract diseases
Respiratory tract infection
Respiratory Tract Infections - epidemiology
Respiratory Tract Infections - virology
RNA, Viral - genetics
Seasons
Subgroups
Viral Envelope Proteins - genetics
Viral Fusion Proteins - genetics
Viral Proteins - genetics
Virology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human metapneumovirus (HMPV) is recognized as a global and frequent cause of acute respiratory tract infections among people of all ages. The objectives of this study were molecular epidemiology and evolutionary analysis of HMPV strains which produced moderate and severe acute respiratory tract infections in children in Croatia during four consecutive seasons (2011‐2014). A total of 117 HMPV‐positive samples collected from hospitalized pediatric patients presenting with acute respiratory tract infections and tested by direct immunofluorescence assay were first analyzed by amplifying a part of the F gene. Sixteen samples were further analyzed based on complete F, G, and SH gene sequences. HMPV genome was identified in 92 of 117 samples (78%) and the circulation of multiple lineages of HMPV was confirmed. In 2011, 2012, and 2014, subgroups A2 and B2 co‐circulated, while B1 gained prevalence in 2013 and 2014. The study established the presence of a novel subcluster A2c in Croatia. This subcluster has only recently been detected in East and Southeast Asia. This study provides new insights into epidemiology and genetic diversity of HMPV in this part of Europe.
ISSN:0146-6615
1096-9071
DOI:10.1002/jmv.24884