Variants of PpuLcc, a multi-dye decolorizing laccase from Pleurotus pulmonarius expressed in Pichia pastoris

• Published in: Protein expression and purification 2017-09, Vol.137, p.34-42
• Main Authors: Behrens, Christoph J., Linke, Diana, Allister, Aldrige B., Zelena, Katerina, Berger, Ralf G.
• Format: Article
• Language: English
• Subjects: beta Carotene - chemistry; Carotenoids - chemistry; Coloring Agents - chemistry; Fungal Proteins - biosynthesis; Fungal Proteins - chemistry; Fungal Proteins - genetics; Fungal Proteins - isolation & purification; Laccase - biosynthesis; Laccase - chemistry; Laccase - genetics; Laccase - isolation & purification; Pichia - chemistry; Pichia - genetics; Pichia - metabolism; Pleurotus - enzymology; Pleurotus - genetics; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - isolation & purification
• ISSN: 1046-5928; 1096-0279
• DOI: 10.1016/j.pep.2017.06.014

A laccase of the basidiomycete Pleurotus pulmonarius (PpuLcc) possessed strong decolorizing abilities towards artificial and natural dyes. The PpuLcc was purified from the culture supernatant via FPLC, and the corresponding gene cloned and expressed in Pichia pastoris GS115. To examine the impact of the C-terminal tail region and the signal peptide on the recombinant expression of PpuLcc, a non-modified version or different truncations (−2, −5, −13 AA) of the target protein were combined with different secretion signals. Heterologous expression of codon optimized constructs resulted in extracellular activities of the PpuLcc variants of up to 7000 U L−1 (substrate ABTS) which was six times higher than non-codon optimized constructs. In contrast to previous works, altering the C-terminal end of the protein did not influence kinetic parameters or the rate of expression. The His-Tag purified enzymes showed high temperature optima (50–70 °C) and thermo stability. All of the recombinant variants degraded triarylmethane and azo dyes. Rapid bleaching of β-carotene (E 160a) and the polyene acid norbixin (E 160b) using a laccase was found for the first time. Thus, the enzyme may be useful in decolorizing unwanted polyene pigments, for example from the processing of cheese, bakery, desserts, ice cream or coloured casings. •Heterologous expression of a newly isolated Laccase reached 7000 U/L.•The enzyme had a high heat stability and a broad pH optima for many substrates.•A range of artificial and natural dyes could efficiently be degraded.•The first laccase being able to degrade carotenes with and without a mediator.