Melatonin synthesis in the human ciliary body triggered by TRPV4 activation: Involvement of AANAT phosphorylation

Melatonin is a substance synthesized in the pineal gland as well as in other organs. This substance is involved in many ocular functions, giving its synthesis in numerous eye structures. Melatonin is synthesized from serotonin through two enzymes, the first limiting step into the synthesis of melato...

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Bibliographic Details
Published in:Experimental eye research 2017-09, Vol.162, p.1-8
Main Authors: Alkozi, Hanan Awad, Perez de Lara, María J., Pintor, Jesús
Format: Article
Language:English
Subjects:
AANAT
Arylalkylamine N-Acetyltransferase - metabolism
Blotting, Western
Calmodulin
Cell Line
Chromatography, High Pressure Liquid
Ciliary body
Ciliary Body - cytology
Ciliary Body - metabolism
Epithelial Cells - cytology
Epithelial Cells - metabolism
Eye
Humans
Melatonin
Melatonin - biosynthesis
Phosphorylation
TRPV Cation Channels - metabolism
TRPV4
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Summary:Melatonin is a substance synthesized in the pineal gland as well as in other organs. This substance is involved in many ocular functions, giving its synthesis in numerous eye structures. Melatonin is synthesized from serotonin through two enzymes, the first limiting step into the synthesis of melatonin being aralkylamine N-acetyltransferase (AANAT). In this current study, AANAT phosphorylation after the activation of TRPV4 was studied using human non-pigmented epithelial ciliary body cells. Firstly, it was necessary to determine the adequate time and dose of the TRPV4 agonist GSK1016790A to reach the maximal phosphorylation of AANAT. An increase of 72% was observed after 5 min incubation with 10 nM GSK (**p 
ISSN:0014-4835
1096-0007
DOI:10.1016/j.exer.2017.06.018