A multifunctional fluorescent aptamer probe for highly sensitive and selective detection of cadmium(II)

We report a highly sensitive and selective strategy for Cd(II) assay using a singly labeled multifunctional probe consisting of a Cd(II)-specific aptamer (CAP), which acted as a recognition element for Cd(II) and a signal reporter. The presence of Cd(II) can induce the conformational switching of th...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2017-08, Vol.409 (21), p.4951-4958
Main Authors: Zhu, Yu-Feng, Wang, Yong-Sheng, Zhou, Bin, Yu, Jun-Hui, Peng, Li-Lan, Huang, Yan-Qin, Li, Xue-Jiao, Chen, Si-Han, Tang, Xian, Wang, Xiao-Feng
Format: Article
Language:English
Subjects:
Analytical Chemistry
Aptamers
Assaying
Biochemistry
Biosensors
Cadmium
Characterization and Evaluation of Materials
Chemical properties
Chemistry
Chemistry and Materials Science
Coils
Detection limits
Fluorescence
Food Science
Laboratory Medicine
Monitoring/Environmental Analysis
Probes (Electronic instruments)
Random coil
Research Paper
Strategy
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Summary:We report a highly sensitive and selective strategy for Cd(II) assay using a singly labeled multifunctional probe consisting of a Cd(II)-specific aptamer (CAP), which acted as a recognition element for Cd(II) and a signal reporter. The presence of Cd(II) can induce the conformational switching of the CAP, accompanied by a change in fluorescence intensity. Thereby, a fluorescence strategy for Cd(II) assay was established. The proposed method has a detection limit of 2.15 nM, which is much lower than the detection limits reported in related literature. This strategy involves only an aptamer probe, and the use of such a G 4 -based quencher avoids the dual labeling of the CAP with fluorophore/quencher units. It is obviously more convenient and economical than the other aptamer-based biosensors for Cd(II) detection. The mechanism by which Cd(II) induces the CAP to change from a random coil sequence to a stem-loop structure was studied in a series of control experiments. This strategy would be helpful in the design of a sensitive analytical platform for various target assays in environmental and biomedical fields. Graphical Abstract The presence of Cd 2+ leads to the conformational change of CAP from a random coil sequence to a stem-loop structure, resulting in a quenching in the fluorescence
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-017-0436-1